Jcowan4 Journal Week 9

Purpose

The purpose of the assignment is to be able to analyze data life cycle for a DNA microarray dataset, to be able to interpret the clusters and associated Gene Ontology terms, to be able to develop an intuition about gene regulatory networks, to be able to keep a detailed electronic laboratory notebook to facilitate reproducible research.

Methods/Results

Analyzing and Interpreting STEM Results

1. 1. Select one of the profiles you saved in the previous step for further intepretation of the data. I suggest that you choose one that has a pattern of up- or down-regulated genes at the cold shock timepoints. Each member of your group should choose a different profile. Answer the following:
      • Why did you select this profile? In other words, why was it interesting to you? I selected my profile of the trend and how it spikes at the 90m mark.
      • How many genes belong to this profile? 266 Genes
      • How many genes were expected to belong to this profile? 27.6 Genes
      • What is the p value for the enrichment of genes in this profile? 5.4E-168 How many GO terms are associated with this profile at p < 0.05? How many GO terms are associated with this profile with a corrected p value < 0.05?

Data and Files

Slide Photos

Acknowledgment and References

• LMU BioDB 2019. (2019). Week 9. Retrieved October 28, 2019 from [https://xmlpipedb.cs.lmu.edu/biodb/fall2019/index.php/Week_9].

Purpose and methods copied/modified from Professor Dahlquist