Ymesfin Week 11
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Contents
- 1 10 Biological Terms
- 2 Outline
- 2.1 What is the main result presented in this paper?
- 2.2 What is the importance or significance of this work?
- 2.3 What were the limitations in previous studies that led them to perform this work?
- 2.4 How did they treat the yeast cells (what experiment were they doing?)
- 2.5 What strain(s) of yeast did they use? Were the strain(s) haploid or diploid?
- 2.6 What media did they grow them in? What temperature? What type of incubator? For how long?
- 2.7 What controls did they use?
- 2.8 How many replicates did they perform per treatment or timepoint?
- 2.9 What method did they use to prepare the RNA, label it and hybridize it to the microarray?
- 2.10 What mathematical/statistical method did they use to analyze the data?
- 2.11 Are the data publicly available for download? From which web site?
- 2.12 Briefly state the result shown in each of the figures and tables, not just the ones you are presenting.
- 2.13 What do the X and Y axes represent?
- 2.14 How were the measurements made?
- 2.15 What trends are shown by the plots and what conclusions can you draw from the data?
- 2.16 How does this work compare with previous studies?
- 2.17 What are the important implications of this work?
- 2.18 What future directions should the authors take?
- 2.19 Give a critical evaluation of how well you think the authors supported their conclusions with the data they showed. Are there any major flaws to the paper
- 3 Annotated Bibliography
- 4 Data/Files
- 5 Conclusion
- 6 Acknowledgements
- 7 References
10 Biological Terms
- homogenization = the process to create a homogenous mixture of a material. (Biology Online, 2019)
- cyclin = a category of proteins that regulate the cell division cycle of eukaryotes (Oxford Dictionary of Biochemistry and Molecular Biology, 2008)
- septins = a category of cytoskeletal proteins associated with GTB Binding protein and creating filaments that are associated with membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling (A Dictionary of Biomedicine, 2010)
- trehalose = a disaccharide consisting of 1-1 α-linkages between glucose molecules (Biology Online, 2019)
- regulon = a system of two genes on different parts of a chromosome that are systematically regulated by the same regulator molecule [(The Dictionary of Cell and Molecular Biology, 2007)]
- reporter gene = a gene that synthesizes an easily detectible protein and is also upstream of the gene of interest. Using such genes allows researchers to easily monitor which variable affect the expression of the target gene (The Dictionary of Cell and Molecular Biology, 2007)
- chemostat = the process by which a bacterial culture is kept in its exponential growth phase by monitoring the concentrations of rate limiting nutrients, media, and cells (A Dictionary of Biomedicine, 2010)
- b-galactosidase = an enzyme encoded by the lacZ gene in the lac operon responsible for the hydrolysis of beta-galactose from the terminal end of sugars (A Dictionary of Biomedicine, 2010)
- recombinant = a portion of spliced DNA out of the strand by restriction enzymes and reintroduced to different regions by ligases (The Dictionary of Cell and Molecular Biology, 2007)
- immunofluorescence = a biochemical method with which a an antibody is linked to a fluorochrome in order to visually detect the presence of a particular antigen A Dictionary of Biomedicine, 2010)
