Difference between revisions of "Nstojan Week 2"

From LMU BioDB 2024
Jump to navigation Jump to search
(added purpose of assignment)
(updated methods)
Line 1: Line 1:
 
*Purpose: The purpose of this assignment is to get a pure-breeding purple plant by constructing a purple protein by understanding how the DNA sequence of a pigment protein gene determines the phenotype of the protein produced.
 
*Purpose: The purpose of this assignment is to get a pure-breeding purple plant by constructing a purple protein by understanding how the DNA sequence of a pigment protein gene determines the phenotype of the protein produced.
  
*Methods:Firstly I launched the Aipotu and switched to the 'Molecular Biology' section near the top of the window. I then went to the greenhouse section and and double clicked the Green-2 organism. I then compared the DNA sequences of the two pigment proteins by clicking on 'Compare' menu and choosing 'Lower vs. Upper'. I then edited the DNA sequence by clicking the 'Fold' button which predicted the color of the protein. I then double-clicked the Red organism located in the Greenhouse and then clicked the top DNA strand at base 20. Once the corresponding bases were selected (highlighted in blue), I typed in'C' which changed the base 20 from G to C. I then clicked on 'Fold Protein' in the Upper Gene Window which folded the protein.The new organism created was saved by clicking 'Add' which was added to the Greenhouse once I named it.
+
*Methods: Firstly I launched the Aipotu website and in the 'Genetics' section, I went to the 'Greenhouse' on the right hand side and self-crossed each of the four original organisms to get all of the colors. Once a new color was discovered I would click on the color and click 'add' above the greenhouse to add the new color. I then switched to the 'Molecular Biology' tab on the right hand side and clicked on the different organisms, then used the 'compare' function on the upper left hand side to compare the DNA sequences. (Insert method to construct a pure-breeding purple organism). Lastly, I went to the 'Biochemistry' tab on the upper left hand side and entered "MLVKEIAMYRFATHER" in the amino acid sequence space and presses 'Fold' to make the purple protein.

Revision as of 23:48, 25 January 2024

  • Purpose: The purpose of this assignment is to get a pure-breeding purple plant by constructing a purple protein by understanding how the DNA sequence of a pigment protein gene determines the phenotype of the protein produced.
  • Methods: Firstly I launched the Aipotu website and in the 'Genetics' section, I went to the 'Greenhouse' on the right hand side and self-crossed each of the four original organisms to get all of the colors. Once a new color was discovered I would click on the color and click 'add' above the greenhouse to add the new color. I then switched to the 'Molecular Biology' tab on the right hand side and clicked on the different organisms, then used the 'compare' function on the upper left hand side to compare the DNA sequences. (Insert method to construct a pure-breeding purple organism). Lastly, I went to the 'Biochemistry' tab on the upper left hand side and entered "MLVKEIAMYRFATHER" in the amino acid sequence space and presses 'Fold' to make the purple protein.