Ckaplan Week 2

Lab report Week 2:

Aipotu II: Biochemistry

Purpose:

The purpose of this Aipotu learning lab: Biochemistry: was to explore the expression of alleles in flowers through self and cross breeding a multitude of colors. The colors of the flowers included red, orange, yellow, green, blue, purple, and black. The job of my lab partner and I was to find the amino acid sequence that coded for and produced a purple flower.

Combined Methods/Results (Electronic Lab Notebook):

• Notes:

Writing the individual letters of the amino acids and clicking fold made each colored allele combination.

• Blue 1 and Red 1 crossed made purple flowers.
• Yellow 1 and Purple 1 crossed made orange.
• Red and green 1 made black
• Green and Red made Purple.
• Yellow and Red made all orange.
• Yellow and white makes all yellow.
• Green and white makes a mix of blue and yellow.

True breeding alleles: Green 1, white, red, yellow, blue- alleles for the colors

True breeding:

CgCg green CrCr red CwCw white CbCb blue CyCy yellow

1. Questions:

a) Which proteins are found in each of the four starting organisms?

• Green 1:MetSerAsnArgHisIleLeuLeuValTyrTrpArgGln
• Green 2: Combination of Blue MetSerAsnArgHisIleLeuLeuValTyrCysArgGln and yellow MetSerAsnArgHisIleLeuLeuValTrpCysArgGln
• Blue: MetSerAsnArgHisIleLeuLeuValTyrCysArgGln
• Yellow: MetSerAsnArgHisIleLeuLeuValTrpCysArgGln
• Red: MetSerAsnArgHisIleLeuLeuValPheCysArgGln
• White: MetSerAsnArgHisIleLeuLeuValValCysArgGln

b) Allele, color, and amino acid sequence

1. Alleles:

• Green
• Blue
• Purple
• Yellow
• White
• Red

1. Colors: same as corresponding alleles

• Green
• Blue
• Purple
• Yellow
• White
• Red

Amino acid sequence: in order of listed colors

• MetSerAsnArgHisIleLeuLeuValTyrTrpArgGln
• MetSerAsnArgHisIleLeuLeuValTyrCysArgGln
• MetSerAsnArgHisIleLeuLeuValTyrPheArgGln
• MetSerAsnArgHisIleLeuLeuValTrpCysArgGln
• MetSerAsnArgHisIleLeuLeuValValCysArgGln
• MetSerAsnArgHisIleLeuLeuValTyrPheArgGln