Data Analysis

Final Project Links
Overview	Deliverables	Guilds	Project Manager	Quality Assurance	Data Analysis	Coder/Designer
		Team	Yeast Beasts

The role of the Data Analyst will be to apply the data analysis pipeline that you learned by analyzing the Dahlquist Lab microarray dataset to complete the analysis of a different published yeast timecourse microarray dataset. The Data Analysts are the end-users of the project, ultimately determining whether the work of the coder/designer and quality assurance members is useful to them.

Guild Members

• Katie & Charlotte

Milestones

The milestones do not necessarily correspond to particular days/weeks; instead they are sets of tasks grouped together.

• Data Analysts can have a shared individual journal entry. Both students will be given the same grade and are expected to contribute equally to the electronic lab notebook.

Milestone 1: Journal Club Presentation

• The Data Analysts will work with their teams to create and deliver a Journal Club presentation about to their team's assigned paper.

Milestone 2: Getting the data ready for analysis

1. Download and examine the microarray dataset, comparing it to the samples and experiment described in your journal club article.
2. Along with the QA's, make a "sample-data relationship table" that lists all of the samples (microarray chips), noting the treatment, time point, and replicate number.
   • Come up with consistent column headers that summarize this information
     • For example, the Dahlquist Lab microarray data used strain_LogFC_timepoint-replicate number, as in wt_LogFC_t15-1.
3. Organize the data in a worksheet in an Excel workbook so that:
   • ID is in the first column
   • Data columns are to the right, in increasing chronological order, using the column header pattern you created
   • Replicates are grouped together

Milestone 3: ANOVA analysis

1. Perform an ANOVA analysis of the data, as you did on Week 9 for the Dahlquist lab data.
   • Note that you will need to adjust your formulas to take into account the different number of timepoints and replicates in your article's dataset.
   • Also note that you will need to consult with Dr. Dahlquist on how to convert the Affymetrix data from the paper into Log2 fold changes.

Milestone 4: Clustering with stem and YEASTRACT

1. Cluster the data with stem, as you did on Week 10.
   • Note that we will make some adjustments to the GO term analysis because stem was not providing GO term names. We are going to use the GO enrichment tool at GeneOntology.org instead.
   1. Go to http://geneontology.org/.
   2. For the cluster you want to analyze, open the gene list and copy the list of genes.
   3. Paste the list of genes into the "Go Enrichment Analysis" box on the right hand side of the GeneOntology.org page.
   4. Select "Saccharomyces cerevisiae" from the species drop-down menu.
   5. Click the "Launch" button.
   6. Near the bottom of the results page, click on the button to Export "Table".
   7. This will prompt you to save a .txt file that can be opened in Excel to view your results.
2. Use YEASTRACT to generate a candidate gene regulatory network as you did on Week 10.

Milestone 5: Create an input workbook for GRNmap using MS Access database

1. Create an input workbook for GRNmap using GRNsight and the Microsoft Access database that the Coder/Designer and QA's make, protocol TBA.
2. Run GRNmap in Dr. Dahlquist's research lab (make appointment) and interpret data.
3. As the end-user of the Access database, the Data Analysts will provide feedback to the QAs and Coder/Designer about the usability of database.

Final Project Links
Overview	Deliverables	Guilds	Project Manager	Quality Assurance	Data Analysis	Coder/Designer
		Team	Yeast Beasts