Difference between revisions of "MSymond1 Week 2"
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The software Aipotu was used for this lab, using the molecular biology tab. The completion of the molecular biology section of this lab did require assistance from the group that completed the genetics portion and the biochemistry portion. They helped provide a list of all different alleles which can be found below, as well as which alleles are true breeding. The molecular biology tab of this software displays the entire DNA and protein sequence of the alleles and what color pigment they make. The first DNA sequence edited in this experiment was the sequence from the red flower. The base that was edited was from the red sequence, and the base was base #20. It was changed from a G to a C. This led the protein sequence to not be folded, because it did not allow for methionine to be coded, therefore, no pigmented protein could be created, making it a white flower. This led to the discovery that there are multiple ways to create a white flower, because any DNA sequence that does not create a functional pigmented protein will create a white flower (figure 1). Using the knowledge of the different alleles, provided by the genetics group, as well as the biochemistry of a purple allele from the biochemistry group, a purple allele for flowers was created using the molecular biology of the organisms to create a true breeding purple organism (figure 2). The three aromatic amino acids that are responsible for color are phenylalanine, tyrosine, and tryptophan. The red sequence from a purple heterozygote had phenylalanine, and the blue sequence had tyrosine. So an amino acid sequence was created that had both of those amino acids. This was done by changing base 82 of a blue sequence to a U, giving it a phenylalanine (figure 3). | The software Aipotu was used for this lab, using the molecular biology tab. The completion of the molecular biology section of this lab did require assistance from the group that completed the genetics portion and the biochemistry portion. They helped provide a list of all different alleles which can be found below, as well as which alleles are true breeding. The molecular biology tab of this software displays the entire DNA and protein sequence of the alleles and what color pigment they make. The first DNA sequence edited in this experiment was the sequence from the red flower. The base that was edited was from the red sequence, and the base was base #20. It was changed from a G to a C. This led the protein sequence to not be folded, because it did not allow for methionine to be coded, therefore, no pigmented protein could be created, making it a white flower. This led to the discovery that there are multiple ways to create a white flower, because any DNA sequence that does not create a functional pigmented protein will create a white flower (figure 1). Using the knowledge of the different alleles, provided by the genetics group, as well as the biochemistry of a purple allele from the biochemistry group, a purple allele for flowers was created using the molecular biology of the organisms to create a true breeding purple organism (figure 2). The three aromatic amino acids that are responsible for color are phenylalanine, tyrosine, and tryptophan. The red sequence from a purple heterozygote had phenylalanine, and the blue sequence had tyrosine. So an amino acid sequence was created that had both of those amino acids. This was done by changing base 82 of a blue sequence to a U, giving it a phenylalanine (figure 3). | ||
*Figure 1 | *Figure 1 | ||
| − | + | [[File:modifiedred.png|200px|thumb|left|alt text]] | |
{{Template:MSymond1}} | {{Template:MSymond1}} | ||
Revision as of 21:34, 24 January 2024
Contents
Methods and Results
The software Aipotu was used for this lab, using the molecular biology tab. The completion of the molecular biology section of this lab did require assistance from the group that completed the genetics portion and the biochemistry portion. They helped provide a list of all different alleles which can be found below, as well as which alleles are true breeding. The molecular biology tab of this software displays the entire DNA and protein sequence of the alleles and what color pigment they make. The first DNA sequence edited in this experiment was the sequence from the red flower. The base that was edited was from the red sequence, and the base was base #20. It was changed from a G to a C. This led the protein sequence to not be folded, because it did not allow for methionine to be coded, therefore, no pigmented protein could be created, making it a white flower. This led to the discovery that there are multiple ways to create a white flower, because any DNA sequence that does not create a functional pigmented protein will create a white flower (figure 1). Using the knowledge of the different alleles, provided by the genetics group, as well as the biochemistry of a purple allele from the biochemistry group, a purple allele for flowers was created using the molecular biology of the organisms to create a true breeding purple organism (figure 2). The three aromatic amino acids that are responsible for color are phenylalanine, tyrosine, and tryptophan. The red sequence from a purple heterozygote had phenylalanine, and the blue sequence had tyrosine. So an amino acid sequence was created that had both of those amino acids. This was done by changing base 82 of a blue sequence to a U, giving it a phenylalanine (figure 3).
- Figure 1
User Page
Assignment Pages
Individual Journal Pages
- MSymond1 Week 1
- MSymond1 Week 2
- MSymond1 KMill104 Week 3
- NeMO_Week4
- MSymond1 Week 5
- MSymond1 Week 6
- MSymond1 Week 8
- MSymond1 Week 9
- MSymond1 Week 10
- MSymond1 Week 12
- MSymond1 Week 13
- MSymond1 Week 15
