Ckaplan Week 2

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Lab report Week 2:

Aipotu II: Biochemistry

Purpose:

The purpose of this Aipotu learning lab: Biochemistry: was to explore the expression of alleles in flowers through self and cross breeding a multitude of colors. The colors of the flowers included red, orange, yellow, green, blue, purple, and black. The job of my lab partner and I was to find the amino acid sequence that coded for and produced a purple flower.

Combined Methods/Results (Electronic Lab Notebook):

  • Notes:

Writing the individual letters of the amino acids and clicking fold made each colored allele combination.

-Blue 1 and Red 1 crossed made purple flowers.

  • Yellow 1 and Purple 1 crossed made orange.
  • Red and green 1 made black

]*Green and Red made Purple.

  • Yellow and Red made all orange.
  • Yellow and white makes all yellow.
  • Green and white makes a mix of blue and yellow.
  • True breeding alleles: Green 1, white, red, yellow, blue- alleles for the colors

True breeding:

CgCg green CrCr red CwCw white CbCb blue CyCy yellow

  1. Questions:

a) Which proteins are found in each of the four starting organisms? Green 1:MetSerAsnArgHisIleLeuLeuValTyrTrpArgGln Green 2: Combination of Blue MetSerAsnArgHisIleLeuLeuValTyrCysArgGln and yellow MetSerAsnArgHisIleLeuLeuValTrpCysArgGln Blue: MetSerAsnArgHisIleLeuLeuValTyrCysArgGln Yellow: MetSerAsnArgHisIleLeuLeuValTrpCysArgGln Red: MetSerAsnArgHisIleLeuLeuValPheCysArgGln White: MetSerAsnArgHisIleLeuLeuValValCysArgGln