Ckaplan Week 2
Contents
Lab report Week 2:
Aipotu II: Biochemistry
Purpose:
The purpose of this Aipotu learning lab: Biochemistry: was to explore the expression of alleles in flowers through self and cross breeding a multitude of colors. The colors of the flowers included red, orange, yellow, green, blue, purple, and black. The job of my lab partner and I was to find the amino acid sequence that coded for and produced a purple flower.
Combined Methods/Results (Electronic Lab Notebook):
- Notes:
Writing the individual letters of the amino acids and clicking fold made each colored allele combination.
- Blue 1 and Red 1 crossed made purple flowers.
- Yellow 1 and Purple 1 crossed made orange.
- Red and green 1 made black
- Green and Red made Purple.
- Yellow and Red made all orange.
- Yellow and white makes all yellow.
- Green and white makes a mix of blue and yellow.
True breeding alleles: Green 1, white, red, yellow, blue- alleles for the colors
True breeding:
CgCg green CrCr red CwCw white CbCb blue CyCy yellow
- Questions:
a) Which proteins are found in each of the four starting organisms?
- Green 1:MetSerAsnArgHisIleLeuLeuValTyrTrpArgGln
- Green 2: Combination of Blue MetSerAsnArgHisIleLeuLeuValTyrCysArgGln and yellow MetSerAsnArgHisIleLeuLeuValTrpCysArgGln
- Blue: MetSerAsnArgHisIleLeuLeuValTyrCysArgGln
- Yellow: MetSerAsnArgHisIleLeuLeuValTrpCysArgGln
- Red: MetSerAsnArgHisIleLeuLeuValPheCysArgGln
- White: MetSerAsnArgHisIleLeuLeuValValCysArgGln
b) Allele, color, and amino acid sequence
- Alleles:
- Green
- Blue
- Purple
- Yellow
- White
- Red
- Colors: same as corresponding alleles
- Green
- Blue
- Purple
- Yellow
- White
- Red
Amino acid sequence: in order of listed colors
- MetSerAsnArgHisIleLeuLeuValTyrTrpArgGln
- MetSerAsnArgHisIleLeuLeuValTyrCysArgGln
- MetSerAsnArgHisIleLeuLeuValTyrPheArgGln
- MetSerAsnArgHisIleLeuLeuValTrpCysArgGln
- MetSerAsnArgHisIleLeuLeuValValCysArgGln
- MetSerAsnArgHisIleLeuLeuValTyrPheArgGln
