Ckaplan Week 2

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Lab report Week 2:

Aipotu II: Biochemistry

Purpose:

The purpose of this Aipotu learning lab: Biochemistry: was to explore the expression of alleles in flowers through self and cross breeding a multitude of colors. The colors of the flowers included red, orange, yellow, green, blue, purple, and black. The job of my lab partner and I was to find the amino acid sequence that coded for and produced a purple flower.

Combined Methods/Results (Electronic Lab Notebook):

  • Notes:

Writing the individual letters of the amino acids and clicking fold made each colored allele combination.

  • Blue 1 and Red 1 crossed made purple flowers.
  • Yellow 1 and Purple 1 crossed made orange.
  • Red and green 1 made black
  • Green and Red made Purple.
  • Yellow and Red made all orange.
  • Yellow and white makes all yellow.
  • Green and white makes a mix of blue and yellow.


True breeding alleles: Green 1, white, red, yellow, blue- alleles for the colors

True breeding:

CgCg green CrCr red CwCw white CbCb blue CyCy yellow

  1. Questions:

a) Which proteins are found in each of the four starting organisms?


  • Green 1:MetSerAsnArgHisIleLeuLeuValTyrTrpArgGln
  • Green 2: Combination of Blue MetSerAsnArgHisIleLeuLeuValTyrCysArgGln and yellow MetSerAsnArgHisIleLeuLeuValTrpCysArgGln
  • Blue: MetSerAsnArgHisIleLeuLeuValTyrCysArgGln
  • Yellow: MetSerAsnArgHisIleLeuLeuValTrpCysArgGln
  • Red: MetSerAsnArgHisIleLeuLeuValPheCysArgGln
  • White: MetSerAsnArgHisIleLeuLeuValValCysArgGln

b) Allele, color, and amino acid sequence

  1. Alleles:
  • Green
  • Blue
  • Purple
  • Yellow
  • White
  • Red
  1. Colors: same as corresponding alleles
  • Green
  • Blue
  • Purple
  • Yellow
  • White
  • Red

Amino acid sequence: in order of listed colors

  • MetSerAsnArgHisIleLeuLeuValTyrTrpArgGln
  • MetSerAsnArgHisIleLeuLeuValTyrCysArgGln
  • MetSerAsnArgHisIleLeuLeuValTyrPheArgGln
  • MetSerAsnArgHisIleLeuLeuValTrpCysArgGln
  • MetSerAsnArgHisIleLeuLeuValValCysArgGln
  • MetSerAsnArgHisIleLeuLeuValTyrPheArgGln