Nstojan1 Week 12
- Make a list of at least 10 biological terms for which you did not know the definitions when you first read the article. Define each of the terms. You can use the glossary in any molecular biology, cell biology, or genetics text book as a source for definitions, or you can use one of many available online biological dictionaries (links below). Cite your sources for the definitions by providing the proper citation (for a book) or the URL to the page with the definition for online sources. Each definition must have it's own citation, to a book or URL. Make an in text citation of the (name, year) format next to the definition, and then list the full citation in the References section of your journal page.
- Blotting: The transfer of protein, rna, dna molecules from a relatively thick acrylamide or agrose gel or to a paper like membrane (usually nylon or agarose gel) by capillarity or an electric field, preserving the spatial arrangment. Once on the membrane, the molecules are immobilised, typically by baking or by ultra violet irradiation and can then be detected at high sensitivity by hybridisation (in the case of dna & rna) or antibody labelling (in the case of protein). Rna blots are called northern blots, dna blots, southern, protein blots, western (Biology Online, 2022).
- Filamentous growth: The process in which a multicellular organism, a unicellular organism or a group of unicellular organisms grow in a threadlike, filamentous shape (AmiGO 2)
- Respiratory electron transport chain: A process in which a series of electron carriers operate together to transfer electrons from donors such as NADH and FADH2 to any of several different terminal electron acceptors to generate a transmembrane electrochemical gradient. (AmiGO 2)
- Answer the following questions about your article. Your answers need to be in YOUR OWN WORDS, not copied straight from the article. It is not acceptable to copy another student's answers either. Even if you work together to understand the article, your individual entries need to be in your own words.
- What is the main result presented in this paper?
- What is the importance or significance of this work?
- What were the limitations in previous studies that led them to perform this work?
- How did they treat the yeast cells (what experiment were they doing?)
- What strain(s) of yeast did they use? Were the strain(s) haploid or diploid?
- What media did they grow them in? What temperature? What type of incubator? For how long?
- What controls did they use?
- How many replicates did they perform per treatment or timepoint?
- What method did they use to prepare the RNA, label it and hybridize it to the microarray?
- What mathematical/statistical method did they use to analyze the data?
- Are the data publicly available for download? From which web site?
- Briefly state the result shown in each of the figures and tables, not just the ones you are presenting.
- What do the X and Y axes represent (if applicable)?
- How were the measurements made?
- What trends are shown by the plots and what conclusions can you draw from the data?
- How does this work compare with previous studies?
- What are the important implications of this work?
- What future directions should the authors take?
- Give a critical evaluation of how well you think the authors supported their conclusions with the data they showed. Are there any major flaws to the paper?