Hivanson Week 13
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- Download and examine the microarray dataset, comparing it to the samples and experiment described in your journal club article.
- Link to processed dataset from SGD.
- For your reference, this is the link to the dataset at the GEO Database:GSE26169. However, we will use the dataset processed by SGD.
- Along with the QA's, make a "sample-data relationship table" that lists all of the samples (microarray chips), noting the treatment, time point, and replicate number.
- Report on these quality measures:
- Are all the samples described in the paper in the dataset?
- Are all the samples in the dataset described in the paper?
- Come up with consistent column headers that summarize this information
- For example, the Dahlquist Lab microarray data used strain_LogFC_timepoint-replicate number, as in wt_LogFC_t15-1.
- Do not use any special characters except for "-" or "_" (e.g., no commas, etc.)
- Organize the data in a worksheet in an Excel workbook so that:
- ID (SGD systematic name) is in the first column
- Data columns are to the right, in increasing chronological order, using the column header pattern you created
- Treatments are grouped together
- Replicates are grouped together
- This information needs to be relayed to the Coder/Designers so that they can design an appropriate
expression
table.