Hivanson Week 13

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  1. Download and examine the microarray dataset, comparing it to the samples and experiment described in your journal club article.
  2. Along with the QA's, make a "sample-data relationship table" that lists all of the samples (microarray chips), noting the treatment, time point, and replicate number.
  3. Report on these quality measures:
    • Are all the samples described in the paper in the dataset?
    • Are all the samples in the dataset described in the paper?
  4. Come up with consistent column headers that summarize this information
    • For example, the Dahlquist Lab microarray data used strain_LogFC_timepoint-replicate number, as in wt_LogFC_t15-1.
    • Do not use any special characters except for "-" or "_" (e.g., no commas, etc.)
  5. Organize the data in a worksheet in an Excel workbook so that:
    • ID (SGD systematic name) is in the first column
    • Data columns are to the right, in increasing chronological order, using the column header pattern you created
    • Treatments are grouped together
    • Replicates are grouped together
  6. This information needs to be relayed to the Coder/Designers so that they can design an appropriate expression table.