Gleis Week 5
From LMU BioDB 2013
E-Notebook
- Obtained copy of Bioinformatics for Dummies Chapter 4
- Read the introduction to Uniprot
- Searched for "P00533" in the query of uniprot.org
- Read the rest of the chapter examining each feature outlined in the chapter
- For the outdated portions of the Chapter I referenced Week 5 which explained some of the changes
Summary
The EGFR is a human receptor tyrosine kinase meaning its catalytic activity is derived from the phosphorylation of a tyrosine. The binding of an appropriate ligand to the protein causes a signal cascade within the cell. EGFR is a membrane spanning protein which enables the protein to bind ligands outside of the membrane and catalyze reactions within the membrane. EGFR is a known oncogene and is believed to play a role in the development of non-small cell lung cancer. One of the expressed domains in EGFR is a membrane helix. The protein is found in tissues throughout the body and plays a role in a large number of cellular processes.
Questions
- The purpose of this exercise was to gain familiarity with the uniprot program while simultaneously concentrating on developing good lab notebook skills.
- From this exercise I learned that I am not very careful with keeping records of my work. Working with UniProt I learned that there is a wealth of information of proteins at Uniprot, even information regarding disease.
- There is such a wealth of information on the site that I have not yet mastered the skills to find the information I am looking for. Additionally I have little knowledge of working with the dozens of tools at the researchers disposal on Uniprot.
