HDelgadi Week 3
Contents |
Complementary Strand
cat seq_file | sed "y/tagc/atcg/"
6 Different Reading Frames
+1 Reading Frame
- cat sequence_file | sed "s/.../& /g" | sed "s/t/u/g" | sed -f genetic-code.sed
+2 Reading Frame
- cat sequence_file | sed "s/^.//g" | sed "s/.../& /g" | sed "s/t/u/g" | sed -f genetic-code.sed
+3 Reading Frame
- cat sequence_file | sed "s/^..//g" | sed "s/.../& /g" | sed "s/t/u/g" | sed -f genetic-code.sed
-1 Reading Frame
- rev sequence_file | sed "y/atgc/tacg/" | sed "s/.../& /g" | sed "s/t/u/g" | sed -f genetic-code.sed
-2 Reading Frame
- rev sequence_file | sed "y/atgc/tacg/" | sed "s/^.//g" | sed "s/.../& /g" | sed "s/t/u/g" | sed -f genetic-code.sed
-3 Reading Frame
- rev sequence_file | sed "y/atgc/tacg/" | sed "s/^..//g" | sed "s/.../& /g" | sed "s/t/u/g" | sed -f genetic-code.sed
XMLPipeDB Match Practice
- What Match command tallies the occurrences of the pattern GO:000916. in the 493.P_falciparum.xml file?
The command: java -jar xmlpipedb-match-1.1.1.jar "GO:000916." <493.P_falciparum.xml.
- How many unique matches are there?
There are two unique matches.
- How many times does each unique match appear?
go:0009165 appears twice and go:0009168 appears once.
- What information do you think the pattern GO:000916. represents?
This pattern might represent the proteins in the organism Falciparum.
- What Match command tallies the occurrences of the pattern \"James.*\" in the 493.P_falciparum.xml file?
The command: java -jar xmlpipedb-match-1.1.1.jar "\"James.*\"" < 493.P_falciparum.xml.
- How many unique matches are there?
There are two unique matches.
- How many times does each unique match appear?
"james k.d." appears 8238 times and "james a.a." appears just once.
- What information do you think the pattern \"James.*\" represents?
This pattern can represent the different labeled proteins.
- Use Match to count the occurrences of the pattern ATG in the hs_ref_GRCh37_chr19.fa file (this may take a while). Then, use grep and wc to do the same thing. What answer does Match give you?
Match gives me 830101 for the occurrences of the pattern ATG in the file.
- What answer does grep/wc give you?
Grep/wc gave me 502410 (lines), 502410 (words), and 35671048 (characters).
- Do the answers make sense? Explain your response.
The answers for Match and grep/wc make sense because grep/wc gives us the number of occurrences in a line and counts them as one even if there's two combined, so it is expected to see a lower number of occurrences through grep/wc than Match.
HDelgadi Week 3
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