Kgosch Week 2

Template:

5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’

Complement:

3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'

Reading Frames

+1: N-Ter[ R(M)LIPCSAYNPAASSAGGIL ]C-Ter (Open Frame)

+2: N-Ter[ VC(STOP)YHVPRITQPPVPLAAF ]C-Ter

+3: N-Ter[ YANT(M)FRV(STOP)PSRQFRWRHF ]C-Ter

-1: N-Ter[ N(STOP)PPRRSRRGLIRRTGYDYA ]C-Ter

-2: N-Ter[ KVATAKVASGIHAKYWLRI ]C-Ter (Open Frame)

-3: N-Ter[ KRRDGQGGVWYAGQV(M)ITH ]C-Ter (Open Frame)

Corrections

The 3' to 5' reading frames should appear as follows:

-1: N-Ter[ (STOP)NAASGTGGWVIRGTWY(STOP)HT ]C-Ter

-2: N-Ter[ K(M)PPAELAAGLYAEHGISI ]C-Ter (Open Frame)

-3: N-Ter[ KCRQRNWRLGYTRN(M)VLAY ]C-Ter (Open Frame)

It turns out that I did correctly separate each group of three in the 3' to 5' direction, however my mistake was not reading the three letters within each group in that direction as well.

Links

Kurt Gosch

Assignment Page

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