Lena Week 2

Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.

5’-cgt atg cta   ata  cca  tgt tcc  gcg tat   aac cca   gcc gcc agt  tcc  gct ggc ggc att  tta-3’
3'-cga tac gat tat ggt aca agg cgc ata ttg ggt cgg cgg tca agg cga ccg cct taa aat-5'

There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence.

+1 R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L
+2 V-C-STOP-Y-H-V-P-R-I-T-Q-P-P-V-P-L-A-A-F
+3 Y-A-N-T-M-F-R-V-STOP-P-S-R-Q-F-R-W-R-H-F

-1 STOP-N-A-A-S-G-T-G-G-W-V-I-R-G-T-W-Y-STOP-H-T
-2 K-M-P-P-A-E-L-A-A-G-L-Y-A-E-H-G-I-S-I
-3 K-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y

Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?

Frames +1, -2, and -3

By Lena Hunt