Difference between revisions of "Lena Week 8"
From LMU BioDB 2013
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===MAPPFinder Procedure=== | ===MAPPFinder Procedure=== | ||
+ | |||
+ | *My Partner and I worked on the '''increased''' criterion. | ||
+ | *From GenMAPP I launched MAPPFinder and opened the expression data set file listed above. I clicked on the "Increaseed" citeria, and checked boxes for "Gene Ontology" and "P Value." |
Revision as of 18:16, 15 October 2013
Contents |
Part 1
- Media: Merrell_Compiled_Raw_Data_Vibrio_LH_20091014.xls
- Media: Merrell_Compiled_Raw_Data_Vibrio_LH_20091014.txt
Part 2
GenMAPP Expression Dataset Manager Procedure
- I used the 2009 version of the Vibrio cholerae Gene Database with GenMAPP.
- I loaded my txt file of Vibrio cholerae, and allowed Expression Dataset Manager to convert the data.
- The processed data was saved here. Media:Merrell_Compiled_Raw_Data_Vibrio_AJV.gex
- The exception file was saved here. Media:Merrell_Compiled_Raw_Data_Vibrio_AJV.EX.txt
- There were 772 errors due to genes not being found.
- I had more errors than my partner who was using the updated version, she has 122. I think the updated version probably had a more comprehensive list of genes for Vibrio cholerae.
- I customized the data set of Avg_LogFC_all with colors. Increased was set to pink and decreased was set to green. To create the criterion for increased I set the Fold Change to > 0.25 and the P Value to < 0.05. To set the criterion for decreased I set Fold Change to < -0.25 and P Value to < 0.05.
- The .gex file was saved here. Media:Merrell_Compiled_Raw_Data_Vibrio_AJV.gex
MAPPFinder Procedure
- My Partner and I worked on the increased criterion.
- From GenMAPP I launched MAPPFinder and opened the expression data set file listed above. I clicked on the "Increaseed" citeria, and checked boxes for "Gene Ontology" and "P Value."