Individual Journal Week 2

User:Emilysimso

• Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand. ( All questions taken from Week 2 assignment)
  • 5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
  • 3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'

• There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
  • 5'-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3' (this is the strand of mRNA copied from the DNA template)
  • 3'-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5' (this is the complementary strand to the mRNA)

• Reading Frames:
  • +1: 5'-cgu aug cua aua cca ugu ucc gcg uau aac cca gcc gcc agu ucc gcu ggc ggc auu uua-3'
    • Amino Acids: R M L I P C S A Y N P A A S S A G G I F
  • +2: 5'-c gua ugc uaa uac cau guu ccg cgu aua acc cag ccg cca guu ccg cug gcg gca uuu ua-3'
    • Amino Acids: V C stop
  • +3: 5'-cg uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cau uuu a-3'
    • Amino Acids: Y A N T M F R V stop
  • -1: 5'-uaa aau gcc gcc agc gga acu ggc ggc ugg guu aua cgc gga acu agg uau uag cau acg-3'
    • Amino Acids: stop N A A S G T G T G G W V I R G T R Y stop
  • -2: 5'-u aaa aug ccg cca gcg gaa cug gcg gcu ggg uua uac gcg gaa cua ggu auu agc aua cg-3'
    • Amino Acids: K M P P A E L A A G L Y A E L G I S I
  • -3: 5'-ua aaa ugc cgc cag cgg aac ugg cgg cug ggu uau acg cgg aac uag gua uua gca uac g-3'
    • Amino Acids: K C R Q R N W R L G Y T R N stop

• Open Reading Frames (do not contain stop codons)
  • +1 and -2 do not contain stop codons