Difference between revisions of "Nanguiano Week 2"

Revision as of 01:14, 11 September 2015

Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.

5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
3’-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’

There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
- In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
- The genetic code is read in the 5’ to 3’ direction.
- Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.

Note: For the full list of codons, see this RNA Codon Table. The stop codon is indicated by "-".
Top, 1

RNA: cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua
Protein: RMLIPCSAYNPAASSAGGIL

RNA: guaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuu
Protein: VC-YHVPRITQPPVPLAAF

RNA: uaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuu
Protein: YANTMFRV-PSRQFRWRHF

Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?
- By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.

@@ Line 10: / Line 10: @@
 ** Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.
+* Note: For the full list of codons, see this [https://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table RNA Codon Table]. The stop codon is indicated by "-".
 * Top, 1
-  RMLIPCSAYNPAASSAGGIL
+  RNA: cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua
+ Protein: RMLIPCSAYNPAASSAGGIL
 * Top, 2
-  gua ugc uaa uac cau guu ccg cgu aua acc cag ccg cca guu ccg cug gcg gca uuu
+  RNA: guaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuu
-  VC-YHVPRITQPPVPLAAF
+ Protein: VC-YHVPRITQPPVPLAAF
 * Top, 3
+ RNA: uaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuu
+ Protein: YANTMFRV-PSRQFRWRHF
 * Bottom, 1
 * Bottom, 2