Difference between revisions of "Nanguiano Week 2"

Revision as of 01:18, 11 September 2015

Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.

5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
3’-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’

There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
- In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
- The genetic code is read in the 5’ to 3’ direction.
- Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.

Note: For the full list of codons, see this RNA Codon Table. The stop codon is indicated by "x".
+1

RNA: 5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’
Protein: N-ter-RMLIPCSAYNPAASSAGGIL-C-ter

RNA: 5’-guaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuu-3’
Protein: N-ter-VCxYHVPRITQPPVPLAAF-C-ter

RNA: 5’-uaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuu-3’
Protein: N-ter-YANTMFRVxPSRQFRWRHF-C-ter

Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?
- By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.

@@ Line 10: / Line 10: @@
 ** Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.
-* Note: For the full list of codons, see this [https://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table RNA Codon Table]. The stop codon is indicated by "-".
+* Note: For the full list of codons, see this [https://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table RNA Codon Table]. The stop codon is indicated by "x".
-* Top, 1
+* +1
-  RNA: cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua
+  RNA: 5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’
-  Protein: RMLIPCSAYNPAASSAGGIL
+  Protein: N-ter-RMLIPCSAYNPAASSAGGIL-C-ter
-* Top, 2
+* +2
-  RNA: guaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuu
+  RNA: 5’-guaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuu-3’
-  Protein: VC-YHVPRITQPPVPLAAF
+  Protein: N-ter-VCxYHVPRITQPPVPLAAF-C-ter
-* Top, 3
+* +3
-  RNA: uaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuu
+  RNA: 5’-uaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuu-3’
-  Protein: YANTMFRV-PSRQFRWRHF
+  Protein: N-ter-YANTMFRVxPSRQFRWRHF-C-ter
-* Bottom, 1
+* -1
-* Bottom, 2
+* -2
-* Bottom, 3
+* -3