Difference between revisions of "Nanguiano Week 2"

Revision as of 01:28, 11 September 2015

Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.

5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
3’-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’

There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
- In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
- The genetic code is read in the 5’ to 3’ direction.
- Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.

Note: For the full list of codons, see this RNA Codon Table. The stop codon is indicated by "x".
+1

RNA: 5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’
Protein: N-ter-RMLIPCSAYNPAASSAGGIL-C-ter

RNA: 5’-guaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuu-3’
Protein: N-ter-VCxYHVPRITQPPVPLAAF-C-ter

RNA: 5’-uaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuu-3’
Protein: N-ter-YANTMFRVxPSRQFRWRHF-C-ter

RNA: 5’-uaaaaugccgccagcggaacuggcggcuggguuauacgcggaacaugguauuagcauacg-3’
Protein: N-ter-xNAASGTGGWVIRGTWYxHT-C-ter

RNA: 5’-aaaaugccgccagcggaacuggcggcuggguuauacgcggaacaugguauuagcaua-3’
Protein: N-ter-KMPPAELAAGLYAEHGISI-C-ter

RNA: 5’-aaaugccgccagcggaacuggcggcuggguuauacgcggaacaugguauuagcauac-3’
Protein: N-ter-KCRQRNWRLGYTRNMVLAY-C-ter

Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?
- By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.