Difference between revisions of "Knguye66 Week 7"

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(Microrray Data Analysis (wild type data): add q)
(Acknowledgements: add wiki sig)
 
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<!--== Notes before starting ==
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* T-test  is this gene expression change significantly different than zero?
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* p<0.05: probability that you would have at least this big of a change by chance
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**ie. when we have 6189 genes, there are 6189 t-tests, are they significant? when we say there's a p<0.05, there's a 5% chance it's due to chance... meaning, if we have 6189 (5% of these are significant just by chance)
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* magnitude of difference
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* variation is sample measurements
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*number of samples/measurements
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* ANOVA  is the gene expression significantly different than zero at any time point?
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This week we will only be doing: experimental design and getting ready
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* starting file: excel (reference this as a link)
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* record file name and time point for data-->
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== Microrray Data Analysis (wild type data) ==
 
== Microrray Data Analysis (wild type data) ==
  
=== Background ===
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==== Purpose ====
 
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This week's assignment is to finish the requirements for "Experimental Design and Getting Ready"
==== Part 1: Statistical Analysis Part 1 ====
 
  
==== Bonferroni and p value Correction ====
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==== Methods/Results ====
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* Download file, re-name, and upload onto wiki page
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* Strain observed: wild-type (wt)
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* Number of replicates: 5 (0-4) for each time point (t15, t30, t60, t90, t120)
  
==== Benjamini & Hochberg p value Correction ====
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==== Data and Files ====
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*File: [[File:WildType_knguye66_Microarray wt.xlsx]]
  
==== Sanity Check ====
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==== Conclusion ====
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N/A
  
* The number of genes that are significantly changed at p value cut-off of p < 0.05:
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== Acknowledgements ==
** How many genes have p < 0.05? and what is the percentage (out of 6189)?
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This section is in acknowledgement to partner Christina Dominguez ([[User:Cdomin12]]), as well as, Marcus Avila ([[User:Mavila9]]) and Jonar Cowan ([[User:Jcowan4]]).
** How many genes have p < 0.01? and what is the percentage (out of 6189)?
 
** How many genes have p < 0.001? and what is the percentage (out of 6189)?
 
** How many genes have p < 0.0001? and what is the percentage (out of 6189)?
 
  
* How many genes are p < 0.05 for the Bonferroni-corrected p value? and what is the percentage (out of 6189)?
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"Except for what is noted above, this individual journal entry was completed by me and not copied from another source." [[User:Knguye66|Knguye66]] ([[User talk:Knguye66|talk]]) 16:11, 16 October 2019 (PDT)
* How many genes are p < 0.05 for the Benjamini and Hochberg-corrected p value? and what is the percentage (out of 6189)?
 
  
* Comparing results with known data. Note that the average Log fold change is what we called "STRAIN)_AvgLogFC_(TIME)" in step 3 of the ANOVA analysis. Does NSR1 change expression due to cold shock in this experiment?
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{{Template:knguye66}}
** Find NSR1 in your dataset. What is its unadjusted, Bonferroni-corrected, and B-H-corrected p values? What is its average Log fold change at each of the timepoints in the experiment?
 
  
* Find your favorite gene from Week 3. What is its unadjusted, Bonferroni-corrected, and B-H-corrected p values? What is its average Log fold change at each of the timepoints in the experiment? Does your favorite gene change expression due to cold shock in this experiment?
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== References ==

Latest revision as of 15:11, 16 October 2019


Microrray Data Analysis (wild type data)

Purpose

This week's assignment is to finish the requirements for "Experimental Design and Getting Ready"

Methods/Results

  • Download file, re-name, and upload onto wiki page
  • Strain observed: wild-type (wt)
  • Number of replicates: 5 (0-4) for each time point (t15, t30, t60, t90, t120)

Data and Files

Conclusion

N/A

Acknowledgements

This section is in acknowledgement to partner Christina Dominguez (User:Cdomin12), as well as, Marcus Avila (User:Mavila9) and Jonar Cowan (User:Jcowan4).

"Except for what is noted above, this individual journal entry was completed by me and not copied from another source." Knguye66 (talk) 16:11, 16 October 2019 (PDT)

User Page

User:knguye66

Template Page

Template:knguye66


Table of all assignments and journal entries for BIO-367-01

Week Individual Journal Entry Shared Journal
Week 1 - Class Journal Week 1
Week 2 knguye66 Week 2 Class Journal Week 2
Week 3 ILT1/YDR090C Week 3 Class Journal Week 3
Week 4 knguye66 Week 4 Class Journal Week 4
Week 5 DrugCentral Week 5 Class Journal Week 5
Week 6 knguye66 Week 6 Class Journal Week 6
Week 7 knguye66 Week 7 Class Journal Week 7
Week 8 knguye66 Week 8 Class Journal Week 8
Week 9 knguye66 Week 9 Class Journal Week 9
Week 10 knguye66 Week 10 Class Journal Week 10
Week 11 knguye66 Week 11 FunGals
Week 12/13 knguye66 Eyoung20 Week 12/13 FunGals
Week 15 knguye66 Eyoung20 Week 15 Class Journal Week 15

References