Difference between revisions of "Individual Journal Page"
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:a) For alleles green and white, there are two differences in amino acids. They are a change from Tyrosine to Valine and from Tryptophan to Cysteine in Green-1 to White respectively. | :a) For alleles green and white, there are two differences in amino acids. They are a change from Tyrosine to Valine and from Tryptophan to Cysteine in Green-1 to White respectively. | ||
[[File:ImacaraePurpleprotein.jpg.png|thumb]] | [[File:ImacaraePurpleprotein.jpg.png|thumb]] | ||
| − | :b) What makes the protein pigmented are the | + | :b) What makes the protein pigmented are the change in Val in the 10th position to a different amino acid. |
| − | :c) What makes the protein a particular color is the change in | + | :c) What makes the protein a particular color is the change in differences in the 10th or 11th amino acid in the sequence. |
:d) Colors combine by adding the amino acid that made them different from another color into the original sequence. | :d) Colors combine by adding the amino acid that made them different from another color into the original sequence. | ||
| Line 32: | Line 32: | ||
*To use mechanics of genetics, biochemistry, molecular biology, and evolution in studying this expression. | *To use mechanics of genetics, biochemistry, molecular biology, and evolution in studying this expression. | ||
| − | ====Methods==== | + | ====Methods and Results==== |
#Downloaded and opened [[http://aipotu.umb.edu/ Aipotu]]. | #Downloaded and opened [[http://aipotu.umb.edu/ Aipotu]]. | ||
| + | #Opened "Biochemistry" tab to view amino acid sequences of Green-1 and White and compare them. | ||
| + | #*Results to ST(a): For alleles green and white, there are two differences in amino acids. They are a change from Tyrosine to Valine and from Tryptophan to Cysteine in Green-1 to White respectively. | ||
| + | |||
#Started on the "Genetics" tab to cross organisms to produce "Purple" organism. | #Started on the "Genetics" tab to cross organisms to produce "Purple" organism. | ||
#*Crossed organism Green-2 to Red. | #*Crossed organism Green-2 to Red. | ||
Revision as of 15:12, 10 September 2019
Contents
Imacarae Week 1
Acknowledgments
- I worked with my homework partner, [Christina Dominguez], in class. We helped each other with the format of editing User and Template pages, as well as helped clarify shared ambiguities of the Week 1 assignment. There was no other contact outside of class or through phone.
- [DeLisa Madere] and I consulted with each other on formatting of pictures and links to pages.
- Except what is noted above, this individual journal entry was completed by me and not copied from another source.
Imacarae (talk) 18:31, 4 September 2019 (PDT)
References
- Instructions for Wiki layout and assignment provided by LMU BioDB 2019. (2019). Week 1. Retrieved August 29, 2017, from [https://xmlpipedb.cs.lmu.edu/biodb/fall2019/index.php/Week_1]
Imacarae Week 2
Biochemistry:
Specific Tasks
- a) For alleles green and white, there are two differences in amino acids. They are a change from Tyrosine to Valine and from Tryptophan to Cysteine in Green-1 to White respectively.
- b) What makes the protein pigmented are the change in Val in the 10th position to a different amino acid.
- c) What makes the protein a particular color is the change in differences in the 10th or 11th amino acid in the sequence.
- d) Colors combine by adding the amino acid that made them different from another color into the original sequence.
- e) The protein found in each of the four original organisms is MSNRHILLV.
- f) The purple protein constructed is sequenced as MSNRHILLVYFCRQ.
Purpose
- To understand how sequences of amino acids affect expression of colors in flowers.
- To use mechanics of genetics, biochemistry, molecular biology, and evolution in studying this expression.
Methods and Results
- Downloaded and opened [Aipotu].
- Opened "Biochemistry" tab to view amino acid sequences of Green-1 and White and compare them.
- Results to ST(a): For alleles green and white, there are two differences in amino acids. They are a change from Tyrosine to Valine and from Tryptophan to Cysteine in Green-1 to White respectively.
- Started on the "Genetics" tab to cross organisms to produce "Purple" organism.
- Crossed organism Green-2 to Red.
- Selected organism 2-8 as "Purple" organism.
- Inserted amino acid Tyr between amino acids Phe and Cys to create purple protein.
- Inserted amino acid Phe between amino acids Tyr and Cyst to create additional protein.
