Difference between revisions of "Individual Journal Page"
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(→Imacarae Week 2: added more methods and results) |
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#*Crossed organism Green-2 to Red. | #*Crossed organism Green-2 to Red. | ||
#*Selected organism 2-8 as "Purple" organism. | #*Selected organism 2-8 as "Purple" organism. | ||
| − | #Inserted amino acid Tyr between amino acids Phe and Cys to create purple protein. | + | #Inserted amino acid Tyr between amino acids Phe and Cys to create purple protein. [[File:ImacaraePurpleprotein.jpg.png|thumb]] |
#Inserted amino acid Phe between amino acids Tyr and Cyst to create additional protein. | #Inserted amino acid Phe between amino acids Tyr and Cyst to create additional protein. | ||
Revision as of 15:17, 10 September 2019
Contents
Imacarae Week 1
Acknowledgments
- I worked with my homework partner, [Christina Dominguez], in class. We helped each other with the format of editing User and Template pages, as well as helped clarify shared ambiguities of the Week 1 assignment. There was no other contact outside of class or through phone.
- [DeLisa Madere] and I consulted with each other on formatting of pictures and links to pages.
- Except what is noted above, this individual journal entry was completed by me and not copied from another source.
Imacarae (talk) 18:31, 4 September 2019 (PDT)
References
- Instructions for Wiki layout and assignment provided by LMU BioDB 2019. (2019). Week 1. Retrieved August 29, 2017, from [https://xmlpipedb.cs.lmu.edu/biodb/fall2019/index.php/Week_1]
Imacarae Week 2
Biochemistry:
- e) The protein found in each of the four original organisms is MSNRHILLV.
- f) The purple protein constructed is sequenced as MSNRHILLVYFCRQ.
Purpose
- To understand how sequences of amino acids affect expression of colors in flowers.
- To use mechanics of genetics, biochemistry, molecular biology, and evolution in studying this expression.
Methods and Results
- Downloaded and opened [Aipotu].
- Opened "Biochemistry" tab to view amino acid sequences of Green-1 and White and compare them.
- Results to ST(a): For alleles green and white, there are two differences in amino acids. They are a change from Tyrosine to Valine and from Tryptophan to Cysteine in Green-1 to White respectively.
- Results to ST(b,c,&d): What makes the protein pigmented are the change in Val in the 10th position to a different amino acid.What makes the protein a particular color is the change in differences in the 10th or 11th amino acid in the sequence. Colors combine by adding the amino acid that made them different from another color into the original sequence.
- Proteins found in the each of the four starting organisms:
- Started on the "Genetics" tab to cross organisms to produce "Purple" organism.
- Crossed organism Green-2 to Red.
- Selected organism 2-8 as "Purple" organism.
- Inserted amino acid Tyr between amino acids Phe and Cys to create purple protein.
- Inserted amino acid Phe between amino acids Tyr and Cyst to create additional protein.
