Difference between revisions of "Jcowan4 Journal Week 11"
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==Acknowledgements== | ==Acknowledgements== | ||
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| + | This week we were put into teams. The team I was assigned to was Team [[Skinny_Genes|Skinny Genes]]. The other members are [[User:Msamdars|Mihir Samdars]], [[User:Ymesfin|Aby Mesfin]], [[User:Dramir36 |David Ramirez]], [[User:Cdomin12|Christina Dominguez]]. We worked in and out of class together Monday and Wednesday. | ||
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| + | "Except for what is noted above, this individual journal entry was completed by me and not copied from another source." | ||
==References== | ==References== | ||
{{jcowan4}} | {{jcowan4}} | ||
(*Participation: Table 1, Table 2, Introduction (Background Information), Question 1, Question 3) | (*Participation: Table 1, Table 2, Introduction (Background Information), Question 1, Question 3) | ||
Revision as of 17:09, 13 November 2019
Contents
- 1 Biological Terms
- 2 Outline
- 2.1 What is the main result presented in this paper?
- 2.2 What is the importance or significance of this work?
- 2.3 What were the limitations in previous studies that led them to perform this work?
- 2.4 How did they treat the yeast cells (what experiment were they doing?)
- 2.5 What strain(s) of yeast did they use? Were the strain(s) haploid or diploid?
- 2.6 What media did they grow them in? What temperature? What type of incubator? For how long?
- 2.7 What controls did they use?
- 2.8 How many replicates did they perform per treatment or timepoint?
- 2.9 What method did they use to prepare the RNA, label it and hybridize it to the microarray?
- 2.10 What mathematical/statistical method did they use to analyze the data?
- 2.11 Are the data publicly available for download? From which web site?
- 2.12 Briefly state the result shown in each of the figures and tables, not just the ones you are presenting.
- 2.13 How does this work compare with previous studies?
- 2.14 What are the important implications of this work?
- 2.15 What future directions should the authors take?
- 2.16 Give a critical evaluation of how well you think the authors supported their conclusions with the data they showed. Are there any major flaws to the paper?
- 3 Annotated Bibliography
- 4 Data/Files
- 5 Conclusion
- 6 Acknowledgements
- 7 References
Biological Terms
Intracellular
Transcriptomic
Extracellular
Methionine
Biosynthesis
B-galactosidase
Homocysteine
Oxidative
Glutathione
Substrate
Transduction
Outline
What is the main result presented in this paper?
The main result found in the article was how the Saccharomyces cerevisiae short-term potassium deprivation causes a lack of potassium which affects and changes sulfur metabolism. This leads to an oxidative stress response and activates the retrograde pathway. It is stated that the catalyst could possibly be ammonium accumulation that happens through the Trk1 potassium transporter. Other results include: a halt in gene expression required for ribosome biogenesis and translation, a decrease in the expression of diverse components used in the progression of cell cycle and blockage in septins assembly. Essentially, a shortage of potassium in the environment triggers an acute transcriptional response, which opens up new finds and more areas to be explored.
What is the importance or significance of this work?
What were the limitations in previous studies that led them to perform this work?
How did they treat the yeast cells (what experiment were they doing?)
What strain(s) of yeast did they use? Were the strain(s) haploid or diploid?
What media did they grow them in? What temperature? What type of incubator? For how long?
- Grown in translucent potassium-free YNB media
- Temperature: 28 Celsius
- Incubator: N/A
- The times used were 0, 10, 20, 40, 60 and 120 minutes
What controls did they use?
How many replicates did they perform per treatment or timepoint?
What method did they use to prepare the RNA, label it and hybridize it to the microarray?
What mathematical/statistical method did they use to analyze the data?
Are the data publicly available for download? From which web site?
Briefly state the result shown in each of the figures and tables, not just the ones you are presenting.
- What do the X and Y axes represent?
- How were the measurements made?
- What trends are shown by the plots and what conclusions can you draw from the data?
How does this work compare with previous studies?
What are the important implications of this work?
What future directions should the authors take?
Give a critical evaluation of how well you think the authors supported their conclusions with the data they showed. Are there any major flaws to the paper?
Annotated Bibliography
Data/Files
Conclusion
Acknowledgements
This week we were put into teams. The team I was assigned to was Team Skinny Genes. The other members are Mihir Samdars, Aby Mesfin, David Ramirez, Christina Dominguez. We worked in and out of class together Monday and Wednesday.
"Except for what is noted above, this individual journal entry was completed by me and not copied from another source."
References
Misc. Links
(*Participation: Table 1, Table 2, Introduction (Background Information), Question 1, Question 3)
