Difference between revisions of "Individual Journal Page"
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(→Imacarae Week 2: added methods 1-2) |
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#*Crossed organism Green-2 to Red. | #*Crossed organism Green-2 to Red. | ||
#*Selected organism 2-8 as "Purple" organism. | #*Selected organism 2-8 as "Purple" organism. | ||
+ | #Inserted amino acid Tyr between amino acids Phe and Cys to create purple protein. | ||
+ | #Inserted amino acid Phe between amino acids Tyr and Cyst to create additional protein. | ||
==Week 3== | ==Week 3== |
Revision as of 14:41, 10 September 2019
Contents
Imacarae Week 1
Acknowledgments
- I worked with my homework partner, [Christina Dominguez], in class. We helped each other with the format of editing User and Template pages, as well as helped clarify shared ambiguities of the Week 1 assignment. There was no other contact outside of class or through phone.
- [DeLisa Madere] and I consulted with each other on formatting of pictures and links to pages.
- Except what is noted above, this individual journal entry was completed by me and not copied from another source.
Imacarae (talk) 18:31, 4 September 2019 (PDT)
References
- Instructions for Wiki layout and assignment provided by LMU BioDB 2019. (2019). Week 1. Retrieved August 29, 2017, from [https://xmlpipedb.cs.lmu.edu/biodb/fall2019/index.php/Week_1]
Imacarae Week 2
Biochemistry:
Specific Tasks
- a) For alleles green and white, there are two differences in amino acids. They are a change from Tyrosine to Valine and from Tryptophan to Cysteine in Green-1 to White respectively.
- b) What makes the protein pigmented are the differences in a difference in the 10th or 11th amino acid in the sequence.
- c) What makes the protein a particular color is the change in Tyrosine to another amino acid.
- d) Colors combine by adding the amino acid that made them different from another color into the original sequence.
- e) The protein found in each of the four original organisms is MSNRHILLV.
- f) The purple protein constructed is sequenced as MSNRHILLVYFCRQ.
Purpose
- To understand how sequences of amino acids affect expression of colors in flowers.
- To use mechanics of genetics, biochemistry, molecular biology, and evolution in studying this expression.
Methods
- Downloaded and opened [Aipotu].
- Started on the "Genetics" tab to cross organisms to produce "Purple" organism.
- Crossed organism Green-2 to Red.
- Selected organism 2-8 as "Purple" organism.
- Inserted amino acid Tyr between amino acids Phe and Cys to create purple protein.
- Inserted amino acid Phe between amino acids Tyr and Cyst to create additional protein.