Difference between revisions of "Knguye66 Week 2"
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| − | | Met Ser Asn Arg His Ile Leu Leu Val Tyr Cys Arg Gln (Blue) | + | | Met Ser Asn Arg His Ile Leu Leu Val {{font color|blue|Tyr}} Cys Arg Gln (Blue) |
| Met Ser Asn Arg His Ile Leu Leu Val Trp Cys Arg Gln (Yellow) | | Met Ser Asn Arg His Ile Leu Leu Val Trp Cys Arg Gln (Yellow) | ||
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| + | '''B)''' | ||
| + | '''C)''' Which features of a protein make it colored? | ||
| + | * The bond structures of a protein make it colored. In the protein, we look for amino acids with altering ring structures, such as that of Phe, Tyr, and Trp. Alternating double and single bonds result in resonance which lowers the wavelength and gives the ability to absorb more pigment and gain more photons. This results in color. | ||
| + | '''D)''' What features of the amino acid sequence make a protein a particular color? | ||
| + | * As observed from the results of the experiment, depending on where the three different aromatic amino acids are placed, Phenylalanine (Phe), Tyrosine (Tyr), and Tryptophan (Trp) are placed or replace another amino acid in the sequence, it results in a different color. For example, to make the color blue, Tyr replaces Val in the "Load Sample Protein" amino acid sequence. To make yellow, Trp replaces Val in the sequence. To make red, Phe replaces Val. A pattern that I have noticed is that these specific amino acids will be strategically in front of Cys. | ||
| − | + | '''E)''' How do the colors combine to produce an overall color? How does this explain the genotype-phenotype rules you found in part (I)? | |
| + | * | ||
== Acknowledgements == | == Acknowledgements == | ||
Revision as of 18:42, 11 September 2019
Contents
Electronic Lab Notebook
- The purpose: what was the purpose of your investigations?
- Your methods: what did you actually do? Give a step by step account.
There should be enough detail provided so that you or another person could re-do it based solely on your notebook. You may copy protocol instructions on your page and modify them as to what you actually did, as long as you provide appropriate attribution. Take advantage of the electronic nature of the notebook by providing screenshots, links to web pages, links to data, etc.
- Your results: the answers to the questions in the protocol, plus any other results you gathered. Usually it makes sense to embed your answers to the questions/results in the "methods" in the order in which you obtained them.
- A scientific conclusion: what was your main finding for today's project? Did you fulfill the purpose? Why or why not?
Results
A) Which proteins are found in each of the four starting organisms?
- Data in the table below:
| Starting Organisms | Upper Folding Window (Allele 1) | Lower Folding Window (Allele 2) |
|---|---|---|
| Green-1 | Met Ser Asn Arg His Ile Leu Leu Val Tyr Trp Arg Gln (Green) | Met Ser Asn Arg His Ile Leu Leu Val Tyr Trp Arg Gln (Green) |
| Green-2 | Met Ser Asn Arg His Ile Leu Leu Val Template:Font color Cys Arg Gln (Blue) | Met Ser Asn Arg His Ile Leu Leu Val Trp Cys Arg Gln (Yellow) |
| Green-Red | Met Ser Asn Arg His Ile Leu Leu Val Phe Cys Arg Gln (Red) | no amino acid sequence found |
| White | Met Ser Asn Arg His Ile Leu Leu Val Val Cys Arg Gln (White) | no amino acid sequence found |
B)
C) Which features of a protein make it colored?
- The bond structures of a protein make it colored. In the protein, we look for amino acids with altering ring structures, such as that of Phe, Tyr, and Trp. Alternating double and single bonds result in resonance which lowers the wavelength and gives the ability to absorb more pigment and gain more photons. This results in color.
D) What features of the amino acid sequence make a protein a particular color?
- As observed from the results of the experiment, depending on where the three different aromatic amino acids are placed, Phenylalanine (Phe), Tyrosine (Tyr), and Tryptophan (Trp) are placed or replace another amino acid in the sequence, it results in a different color. For example, to make the color blue, Tyr replaces Val in the "Load Sample Protein" amino acid sequence. To make yellow, Trp replaces Val in the sequence. To make red, Phe replaces Val. A pattern that I have noticed is that these specific amino acids will be strategically in front of Cys.
E) How do the colors combine to produce an overall color? How does this explain the genotype-phenotype rules you found in part (I)?
Acknowledgements
- This week I worked with Michael Armas (User:Marmas) for the Aipotu II: Biochemistry assignment.
Except for what is noted above, this individual journal entry was completed by me and not copied from another source. Knguye66 (talk) 15:04, 10 September 2019 (PDT)
References
User Page
User:knguye66
Template Page
Template:knguye66
