FAS2 Week 3

Gene Info

Name

Standard Name: FAS2

Systematic Name: YPL231W

Name Description: Fatty Acid Synthetase

Gene ID

SGD ID: S000006152

NCBI ID: 855845

Ensembl ID: YPL231W

Uniprot ID: P19097

DNA Sequence

ATGAAGCCGGAAGTTGAGCAAGAATTAGCTCATATTTTGCTAACTGAATTGTTAGCTTAT CAATTTGCCTCTCCTGTGAGATGGATTGAAACTCAAGATGTTTTTTTGAAGGATTTTAAC ACTGAAAGGGTTGTTGAAATCGGTCCTTCTCCAACTTTGGCTGGGATGGCTCAAAGAACC TTGAAGAATAAATACGAATCTTACGATGCTGCTCTGTCTTTACATAGAGAAATCTTATGC TATTCGAAGGATGCCAAAGAGATTTATTATACCCCAGATCCATCCGAACTAGCTGCAAAG GAAGAGCCCGCTAAGGAAGAAGCTCCTGCTCCAACTCCAGCTGCTAGTGCTCCTGCTCCT GCAGCAGCAGCCCCAGCTCCCGTCGCGGCAGCAGCCCCAGCTGCAGCAGCTGCTGAGATT GCCGATGAACCTGTCAAGGCTTCCCTATTGTTGCACGTTTTGGTTGCTCACAAGTTGAAG AAGTCGTTAGATTCCATTCCAATGTCCAAGACAATCAAAGACTTGGTCGGTGGTAAATCT ACAGTCCAAAATGAAATTTTGGGTGATTTAGGTAAAGAATTTGGTACTACTCCTGAAAAA CCAGAAGAAACTCCATTAGAAGAATTGGCAGAAACTTTCCAAGATACCTTCTCTGGAGCA TTGGGTAAGCAATCTTCCTCGTTATTATCAAGATTAATCTCATCTAAGATGCCTGGTGGG TTTACTATTACTGTCGCTAGAAAATACTTACAAACTCGCTGGGGACTACCATCTGGTAGA CAAGATGGTGTCCTTTTGGTAGCTTTATCTAACGAGCCTGCTGCTCGTCTAGGTTCTGAA GCTGATGCCAAGGCTTTCTTGGACTCCATGGCTCAAAAATACGCTTCCATTGTTGGTGTT GACTTATCATCAGCTGCTAGCGCTAGTGGTGCTGCCGGTGCAGGTGCTGCTGCCGGTGCA GCTATGATCGATGCTGGCGCTCTGGAAGAAATAACCAAAGACCACAAGGTTTTGGCGCGT CAACAACTGCAAGTATTGGCTCGTTATCTAAAAATGGACTTGGATAACGGTGAAAGAAAG TTCTTGAAAGAAAAGGACACTGTTGCTGAACTTCAAGCTCAGTTGGATTACTTGAATGCC GAATTAGGTGAATTCTTTGTTAACGGTGTTGCTACTTCTTTCTCTAGAAAAAAGGCCAGA ACCTTCGATTCTTCCTGGAACTGGGCTAAACAATCTTTATTATCATTATACTTTGAGATA ATTCATGGTGTCTTGAAAAACGTTGATAGAGAGGTTGTTAGTGAAGCTATCAATATCATG AACAGATCTAACGATGCTTTGATTAAATTCATGGAATACCATATCTCTAACACTGATGAA ACAAAAGGTGAAAACTATCAATTGGTTAAAACTCTTGGTGAGCAGTTGATTGAAAACTGT AAACAAGTTTTGGATGTTGATCCAGTTTACAAAGATGTTGCTAAGCCTACCGGTCCAAAA ACTGCTATTGACAAGAACGGTAACATTACATACTCAGAAGAGCCAAGAGAAAAGGTTAGG AAATTATCTCAATACGTACAAGAAATGGCCCTTGGTGGTCCAATCACCAAAGAATCTCAA CCTACTATTGAAGAGGATTTGACTCGTGTTTACAAGGCAATCAGTGCTCAAGCTGATAAA CAAGATATTTCCAGCTCCACCAGGGTTGAATTTGAAAAACTATATAGTGATTTGATGAAG TTCTTGGAAAGCTCCAAAGAAATCGATCCTTCTCAAACAACCCAATTGGCCGGTATGGAT GTTGAGGATGCTTTGGACAAAGATTCCACCAAAGAAGTTGCTTCTTTGCCAAACAAATCT ACCATTTCTAAGACGGTATCTTCAACTATTCCAAGAGAAACTATTCCGTTCTTACATTTG AGAAAGAAGACTCCTGCCGGAGATTGGAAATATGACCGCCAATTGTCTTCTCTTTTCTTA GATGGTTTAGAAAAGGCTGCCTTCAACGGTGTCACCTTCAAGGACAAATACGTCTTGATC ACTGGTGCTGGTAAGGGTTCTATTGGTGCTGAAGTCTTGCAAGGTTTGTTACAAGGTGGT GCTAAGGTTGTTGTTACCACCTCTCGTTTCTCTAAGCAAGTTACAGACTACTACCAATCC ATTTACGCCAAATATGGTGCTAAGGGTTCTACTTTGATTGTTGTTCCATTCAACCAAGGT TCTAAGCAAGACGTTGAAGCTTTGATTGAATTTATCTACGACACTGAAAAGAATGGTGGT TTAGGTTGGGATCTAGATGCTATTATTCCATTCGCGGCCATTCCAGAACAAGGTATTGAA TTAGAACATATTGATTCTAAGTCTGAATTTGCTCATAGAATCATGTTGACCAATATCTTA AGAATGATGGGTTGTGTCAAGAAGCAAAAATCTGCAAGAGGTATTGAAACAAGACCAGCT CAAGTCATTCTACCAATGTCTCCAAACCATGGTACTTTCGGTGGTGATGGTATGTATTCA GAATCCAAGTTGTCTTTGGAAACTTTGTTCAACAGATGGCACTCTGAATCCTGGGCCAAT CAATTAACCGTTTGCGGTGCTATTATTGGTTGGACTAGAGGTACTGGTTTAATGAGCGCT AATAACATCATTGCTGAAGGCATTGAAAAGATGGGTGTTCGTACTTTCTCTCAAAAGGAA ATGGCTTTCAACTTATTGGGTCTATTGACTCCAGAAGTCGTAGAATTGTGCCAAAAATCA CCTGTTATGGCTGACTTGAATGGTGGTTTGCAATTTGTTCCTGAATTGAAGGAATTCACT GCTAAATTGCGTAAAGAGTTGGTTGAAACTTCTGAAGTTAGAAAGGCAGTTTCCATCGAA ACTGCTTTGGAGCATAAGGTTGTCAATGGCAATAGCGCTGATGCTGCATATGCTCAAGTC GAAATTCAACCAAGAGCTAACATTCAACTGGACTTCCCAGAATTGAAACCATACAAACAG GTTAAACAAATTGCTCCCGCTGAGCTTGAAGGTTTGTTGGATTTGGAAAGAGTTATTGTA GTTACCGGTTTTGCTGAAGTCGGCCCATGGGGTTCGGCCAGAACAAGATGGGAAATGGAA GCTTTTGGTGAATTTTCGTTGGAAGGTTGCGTTGAAATGGCCTGGATTATGGGCTTCATT TCATACCATAACGGTAATTTGAAGGGTCGTCCATACACTGGTTGGGTTGATTCCAAAACA AAAGAACCAGTTGATGACAAGGACGTTAAGGCCAAGTATGAAACATCAATCCTAGAACAC AGTGGTATCAGATTGATCGAACCAGAGTTATTCAATGGTTACAACCCAGAAAAGAAGGAA ATGATTCAAGAAGTCATTGTCGAAGAAGACTTGGAACCATTTGAGGCTTCGAAGGAAACT GCCGAACAATTTAAACACCAACATGGTGACAAAGTGGATATCTTCGAAATCCCAGAAACA GGAGAGTACTCTGTTAAGTTACTAAAGGGTGCCACTTTATACATTCCAAAGGCTTTGAGA TTTGACCGTTTGGTTGCAGGTCAAATTCCAACTGGTTGGAATGCTAAGACTTATGGTATC TCTGATGATATCATTTCTCAGGTTGACCCAATCACATTATTCGTTTTGGTCTCTGTTGTG GAAGCATTTATTGCATCTGGTATCACCGACCCATACGAAATGTACAAATACGTACATGTT TCTGAGGTTGGTAACTGTTCTGGTTCTGGTATGGGTGGTGTTTCTGCCTTACGTGGTATG TTTAAGGACCGTTTCAAGGATGAGCCTGTCCAAAATGATATTTTACAAGAATCATTTATC AACACCATGTCCGCTTGGGTTAATATGTTGTTGATTTCCTCATCTGGTCCAATCAAGACA CCTGTTGGTGCCTGTGCCACATCCGTGGAATCTGTTGACATTGGTGTAGAAACCATCTTG TCTGGTAAGGCTAGAATCTGTATTGTCGGTGGTTACGATGATTTCCAAGAAGAAGGCTCC TTTGAGTTCGGTAACATGAAGGCCACTTCCAACACTTTGGAAGAATTTGAACATGGTCGT ACCCCAGCGGAAATGTCCAGACCTGCCACCACTACCCGTAACGGTTTTATGGAAGCTCAA GGTGCTGGTATTCAAATCATCATGCAAGCTGATTTAGCTTTGAAGATGGGTGTGCCAATT TACGGTATTGTTGCCATGGCTGCTACCGCCACCGATAAGATTGGTAGATCTGTGCCAGCT CCAGGTAAGGGTATTTTAACCACTGCTCGTGAACACCACTCCAGTGTTAAGTATGCTTCA CCAAACTTGAACATGAAGTACAGAAAGCGCCAATTGGTTACTCGTGAAGCTCAGATTAAA GATTGGGTAGAAAACGAATTGGAAGCTTTGAAGTTGGAGGCCGAAGAAATTCCAAGCGAA GACCAAAACGAGTTCTTACTTGAACGTACCAGAGAAATCCACAACGAAGCTGAAAGTCAA TTGAGAGCTGCACAACAACAATGGGGTAACGACTTCTACAAGAGGGACCCACGTATTGCT CCATTGAGAGGAGCACTGGCTACTTACGGTTTAACTATTGATGACTTGGGTGTCGCTTCA TTCCACGGTACATCCACAAAGGCTAATGACAAGAACGAATCTGCCACAATTAATGAAATG ATGAAGCATTTGGGTAGATCTGAAGGTAATCCCGTCATTGGTGTTTTCCAAAAGTTCTTG ACTGGTCATCCAAAGGGTGCTGCTGGTGCATGGATGATGAATGGTGCTTTGCAAATTCTA AACAGTGGTATTATTCCAGGTAACCGTAACGCTGATAACGTGGATAAGATCTTGGAGCAA TTTGAATACGTCTTGTACCCATCCAAGACTTTAAAGACCGACGGTGTCAGAGCCGTGTCC ATCACTTCTTTCGGTTTTGGTCAAAAGGGTGGTCAAGCTATTGTGGTTCATCCAGACTAC TTATACGGTGCTATCACTGAAGACAGATACAACGAGTATGTCGCCAAGGTTAGTGCCAGA GAGAAAAGTGCCTACAAATTCTTCCATAATGGTATGATCTACAACAAGTTGTTCGTAAGT AAAGAGCATGCTCCATACACTGATGAATTGGAAGAGGATGTTTACTTGGACCCATTAGCC CGTGTATCTAAGGATAAGAAATCAGGCTCCTTGACTTTCAACTCTAAAAACATCCAAAGC AAGGACAGTTACATCAATGCTAACACCATTGAAACTGCCAAGATGATTGAAAACATGACC AAGGAGAAAGTCTCTAACGGTGGCGTCGGTGTAGATGTTGAATTAATCACTAGCATCAAC GTTGAAAATGATACTTTTATCGAGCGCAATTTCACCCCGCAAGAAATAGAGTACTGCAGC GCGCAGCCTAGTGTGCAAAGCTCTTTCGCTGGGACATGGTCCGCCAAAGAGGCTGTTTTC AAGTCCTTAGGCGTCAAGTCCTTAGGCGGTGGTGCTGCATTGAAAGACATCGAAATCGTA CGCGTTAACAAAAACGCTCCAGCCGTTGAACTGCACGGTAACGCCAAAAAGGCTGCCGAA GAAGCTGGTGTTACCGATGTGAAGGTATCTATTTCTCACGATGACCTCCAAGCTGTCGCG GTCGCCGTTTCTACTAAGAAATAG

Protein Sequence

MKPEVEQELAHILLTELLAYQFASPVRWIETQDVFLKDFNTERVVEIGPSPTLAGMAQRT LKNKYESYDAALSLHREILCYSKDAKEIYYTPDPSELAAKEEPAKEEAPAPTPAASAPAP AAAAPAPVAAAAPAAAAAEIADEPVKASLLLHVLVAHKLKKSLDSIPMSKTIKDLVGGKS TVQNEILGDLGKEFGTTPEKPEETPLEELAETFQDTFSGALGKQSSSLLSRLISSKMPGG FTITVARKYLQTRWGLPSGRQDGVLLVALSNEPAARLGSEADAKAFLDSMAQKYASIVGV DLSSAASASGAAGAGAAAGAAMIDAGALEEITKDHKVLARQQLQVLARYLKMDLDNGERK FLKEKDTVAELQAQLDYLNAELGEFFVNGVATSFSRKKARTFDSSWNWAKQSLLSLYFEI IHGVLKNVDREVVSEAINIMNRSNDALIKFMEYHISNTDETKGENYQLVKTLGEQLIENC KQVLDVDPVYKDVAKPTGPKTAIDKNGNITYSEEPREKVRKLSQYVQEMALGGPITKESQ PTIEEDLTRVYKAISAQADKQDISSSTRVEFEKLYSDLMKFLESSKEIDPSQTTQLAGMD VEDALDKDSTKEVASLPNKSTISKTVSSTIPRETIPFLHLRKKTPAGDWKYDRQLSSLFL DGLEKAAFNGVTFKDKYVLITGAGKGSIGAEVLQGLLQGGAKVVVTTSRFSKQVTDYYQS IYAKYGAKGSTLIVVPFNQGSKQDVEALIEFIYDTEKNGGLGWDLDAIIPFAAIPEQGIE LEHIDSKSEFAHRIMLTNILRMMGCVKKQKSARGIETRPAQVILPMSPNHGTFGGDGMYS ESKLSLETLFNRWHSESWANQLTVCGAIIGWTRGTGLMSANNIIAEGIEKMGVRTFSQKE MAFNLLGLLTPEVVELCQKSPVMADLNGGLQFVPELKEFTAKLRKELVETSEVRKAVSIE TALEHKVVNGNSADAAYAQVEIQPRANIQLDFPELKPYKQVKQIAPAELEGLLDLERVIV VTGFAEVGPWGSARTRWEMEAFGEFSLEGCVEMAWIMGFISYHNGNLKGRPYTGWVDSKT KEPVDDKDVKAKYETSILEHSGIRLIEPELFNGYNPEKKEMIQEVIVEEDLEPFEASKET AEQFKHQHGDKVDIFEIPETGEYSVKLLKGATLYIPKALRFDRLVAGQIPTGWNAKTYGI SDDIISQVDPITLFVLVSVVEAFIASGITDPYEMYKYVHVSEVGNCSGSGMGGVSALRGM FKDRFKDEPVQNDILQESFINTMSAWVNMLLISSSGPIKTPVGACATSVESVDIGVETIL SGKARICIVGGYDDFQEEGSFEFGNMKATSNTLEEFEHGRTPAEMSRPATTTRNGFMEAQ GAGIQIIMQADLALKMGVPIYGIVAMAATATDKIGRSVPAPGKGILTTAREHHSSVKYAS PNLNMKYRKRQLVTREAQIKDWVENELEALKLEAEEIPSEDQNEFLLERTREIHNEAESQ LRAAQQQWGNDFYKRDPRIAPLRGALATYGLTIDDLGVASFHGTSTKANDKNESATINEM MKHLGRSEGNPVIGVFQKFLTGHPKGAAGAWMMNGALQILNSGIIPGNRNADNVDKILEQ FEYVLYPSKTLKTDGVRAVSITSFGFGQKGGQAIVVHPDYLYGAITEDRYNEYVAKVSAR EKSAYKFFHNGMIYNKLFVSKEHAPYTDELEEDVYLDPLARVSKDKKSGSLTFNSKNIQS KDSYINANTIETAKMIENMTKEKVSNGGVGVDVELITSINVENDTFIERNFTPQEIEYCS AQPSVQSSFAGTWSAKEAVFKSLGVKSLGGGAALKDIEIVRVNKNAPAVELHGNAKKAAE EAGVTDVKVSISHDDLQAVAVAVSTKK*

Why This Gene?

We chose this gene after reading an article that summarized the findings of an interesting study on yeast genes that impart flavor in beer and wine during the brewing process. This particular gene was one of two genes that were identified as producing higher levels of a compound called phenylethyl acetate, which imparts a flavor reminiscent of rose or honey (de Carvalho et al., 2017). FAS2 was one of those two genes; it is responsible for encoding the alpha subunit of fatty acid synthase, an enzyme that is unsurprisingly involved in fatty acid synthesis. We thought it would be interesting to explore this gene as it is also essential for the synthesis of essential fatty acids, such as palmitic acid, which is the most common fatty acid present in eukaryotes.

Acknowledgements

This week, the two partners for this project are Mihir Samdarshi and Jonar Cowan. Both partners worked with each other outside of class to assist each other in finding and synthesizing the necessary information required to complete this assignment.

Except for what is noted above, this individual journal entry was completed by me and not copied from another source.

References

1. Cherry, J. M., Hong, E. L., Amundsen, C., Balakrishnan, R., Binkley, G., Chan, E. T., ... & Fisk, D. G. (2011). Saccharomyces Genome Database: the genomics resource of budding yeast. Nucleic Acids Research, 40(D1), D700-D705.
2. Cunningham, F., Amode, M. R., Barrell, D., Beal, K., Billis, K., Brent, S., ... & Gil, L. (2014). Ensembl 2015. Nucleic Acids Research, 43(D1), D662-D669.
3. The UniProt Consortium. (2017). UniProt: the universal protein knowledgebase. Nucleic Acids Research, 45(D1), D158–D169, https://doi.org/10.1093/nar/gkw1099
4. Brown, G. R., Hem, V., Katz, K. S., Ovetsky, M., Wallin, C., Ermolaeva, O., ... & Murphy, T. D. (2014). Gene: a gene-centered information resource at NCBI. Nucleic Acids Research, 43(D1), D36-D42.
5. Researchers identify the yeast genes behind rose and honeyed flavors in beer and wine (2017, November 7) retrieved 16 September 2019 from https://phys.org/news/2017-11-yeast-genes-rose- honeyed-flavors.html
6. de Carvalho, B. T., Holt, S., Souffriau, B., Brandão, R. L., Foulquié-Moreno, M. R., & Thevelein, J. M. (2017). Identification of novel alleles conferring superior production of rose flavor phenylethyl acetate using polygenic analysis in yeast. MBio, 8(6), e01173-17.