Marmas Week 2
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Contents
Purpose
- Identify the differences in amino acid sequences of alleles.
- Identify what features of amino acid sequences of a protein are associated with pigment and color.
- Explain how genotype-phenotype rules apply to how how colors combine to produce a new color.
- Identify what proteins are present in each of the four starting organisms.
- Construct a purple protein using the information gathered of the biochemistry of plant color.
Methods
- Compare the proteins in the starting strains and compare the differences in allele, color, and amino acid sequence.
- Compare the particular colors' protein sequence to the "sample protein"
Results
a) Proteins Produced in each of the four starting organisms:
| Starting Organism | Protein Produced by Allele 1 | Protein Produced by Allele 2 |
|---|---|---|
| Green-1 | Green-Colored Protein | Green Colored Protein |
| Green-2 | Blue-Colored Protein | Yellow-Colored Protein |
| Red | Red-Colored Protein | None |
| White | White-Colored Protein | White Colored Protein |
c) The features of the protein that specifically alter its color are the 10th or 11th amino acid in the sequence. This is not necessarily due to polar or charged characteristics, as changing the polar-uncharged Y10 in the Green-1 organism to a Serine, for example, does not maintain the green color. By modifying either the 10th or 11th protein in the sequence, the color will change. However, if the amino acid sequence is modified in a way that the configuration of the protein is altered, the default color is white.
