Eyoung20 week 2

From LMU BioDB 2019
Revision as of 16:52, 11 September 2019 by Eyoung20 (talk | contribs) (Part 2: added step 5)
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user:eyoung20 week 2

Purpose

The puupose of this experiment is to use the Aipotu software to become more familiar with how genes effect the physical characteristics of organisms. This was done by determining the genes of the flowers of the software and how dominant and recessive properties effect the color, and how mutations can change them.

Methods and results

Part 1

  1. the first step was to run a cross breading experiment on the flower organisms. This experiment allowed the use of the software to determine how many different colors could be made by the flowers.
    • The results of the crosses where:
      • Green 1 - Green 2 : Green
      • Green 1 - Red: Green, Black
      • Green 1 - White: green
      • Green 2 - red: purple, orange, yellow, blue
      • Green 2 - white: blue, yellow
      • Red - white: Red, White
  2. The next step is two self cross all the colors we found using the software by selecting colors from the history trays and hitting the self-cross option. Green 1 and Green 2 are still being treated as two different colors due to the above results showing different results when they are combined with thew other colors.
    • the results of the Self-crosses were:
      • Green 1: Green Eyoung20Green 1 self cross.png
      • Green 2: Yellow, blue, green
      • Red: white, red
      • white: white
      • Yellow: yellow, white
      • Orange: yellow, red, orange
      • Purple: red, blue, purple
      • Blue: Blue, white
      • Black: Green. red, black
  3. The next step was to see if we could achieve a pure bred flower of each of the colors. This was done by self-crossing one flower of the desired color and then repeatedly self-crossing a flower from the results of the desired color. This process should be repeated two to three times, and the final result should be chosen from a result group that is purely the desired color. The pure bred flower is then added to the greenhouse for later use. Not all of the colors where expected to be able to pure breed based on the earlier results.
    • The Pure breeding results:
      • Green 1: will pure breed
      • Green 2: will not pure breed
      • Red: will pure breed
      • White: will pure breed
      • Blue: will pure breed
      • Yellow: will pure breed
      • Black: will not pure breed
      • Orange: will not pure breed
      • Purple: will not pure breed
  4. the next step was to figure out exactly which pure breeding colors when combined were able to make the non pure breeding or heterozyogous colors. This was done by crossing different combinations of different pure breed flowers that were saved to the greenhouse in the above step.
    • The Results
      • Green pure - red pure: Black
      • Green pure - blue pure: Green
      • Green pure - yellow pure:Green
      • Green pure - white pure: Green
      • Red Pure - blue pure: Purple
      • Red Pure - Yellow Pure: Orange
      • Red pure - white pure: Red
      • Blue Pure - Yellow pure: Green
      • Blue Pure - white pure: Blue
      • Yellow pure - white pure: Yellow

=Scientific Conclusion

Based on this data we can answer the questions and tasks that were proposed in the hand out for this experiment.

Part 2

  1. Selected a pure breeding colored flower we isolated in part 1, in this case the white pure breeding flower.
  2. The Pure white was then selected and mutated. the result were one red flower and eleven white flowers. So there was one mutation in this round.
  3. The mutant Red flower was then saved to the Green House as "Red from White"
  4. Then we moved from the Genetics tab in Aipotu to the Biochemistry tab.
  5. The mutant organism was then double clicked so that they two portiens produced by the alless then show up, one in each of the windows.

Scientific Conclusion

questions

Acknowledgements

Reference