Difference between revisions of "Ajvree Week 10"

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*information needed: a reference genome for species/main reference strand, usually the earliest date
 
*information needed: a reference genome for species/main reference strand, usually the earliest date
  
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===Search Results:===
 +
==Article 1:==
 +
*Searched Web of Science for '''transcriptional profiling Streptococcus pneumoniae''' under topic, got 38 results.
 +
*Found article titled : '''Gene expression profile of early in vitro biofilms of Streptococcus pneumoniae''' (Mukesh Kumar Yadav et al., 2012)
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*Used Google Scholar to search for full article
 +
*Article PDF involves comparing microarray data of S. pneumoniae biofilm to planktonic cells. 21 biological microarray replicates were done, with the control being the planktonic cells.
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*Searched article for location of microarray data, located here: '''http://www.ncbi.nlm.nih.gov/geo''' under
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accession number '''GSE34751'''
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 +
*'''PDF:''' [[Media:Geneexpressionarticle1.pdf|S. pneumoniae Article 1]]
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*Full Citation: <br>
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Yadav, M. K., Kwon, S. K., Cho, C. G., Park, S.-W., Chae, S.-W. and Song, J.-J. (2012), Gene expression profile of early in vitro biofilms of Streptococcus pneumoniae. Microbiology and Immunology, 56: 621–629.
 +
*Based on this report and other titles listed while searching, a lot of research regarding vaccines against the pathogenic strain is popular, in addition to research on how to prevent the bacteria from growing.
  
 +
==Article 2:==
 +
*Found second article using references from first article, looked for key words in title about transcription activity/microarray data
 +
*link to article: [http://jb.asm.org/content/185/1/359.full]
 +
*Full Citation:
 +
Ng, WL, Kazmierczak, KM Robertson, GT, et al., 2003. ''Transcriptional regulation and signature patterns revealed by microarray analyses of Streptococcus pneumoniae R6 challenged with sublethal concentrations of translation inhibitors.'' Journal of Bacteriology. Volume 185, Issue 1, Pages 359-370.
 +
*In this experiment, cell cultures were exposed to 4 different translation inhibitors to determine the effect on the transcript activity. The control used was a 1% DMSO culture that contained no antibiotics. There were 3 replicates done of the microarray analysis, and Northern blot analysis was also used.
  
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==Links==
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{{Team ATK}}
  
 
Individual HW:
 
Potential articles:
 
*http://www.ncbi.nlm.nih.gov/pubmed/11463916
 
 
 
[[User:Ajvree|User Page]]
 
 
[[Category:Journal Entry]]
 
[[Category:Journal Entry]]

Latest revision as of 18:15, 18 November 2013

10/29/13 Lecture Notes

  • Library homepage>Libguides>link to BIO 367>databases
  • Open access: physics(peer sharing) and life sciences (availability of granted research)
  • PMC Journal: information designated by govt to be available to all
  • Now authors pay journal to have information published, makes open access controversial
  • http://libguides.lmu.edu/BIOL367
  • Google scholar ranks articles, dates will be all over the place, etc.
  • MeSH terms: medical subheading
  • information needed: a reference genome for species/main reference strand, usually the earliest date

Contents

[edit] Search Results:

[edit] Article 1:

  • Searched Web of Science for transcriptional profiling Streptococcus pneumoniae under topic, got 38 results.
  • Found article titled : Gene expression profile of early in vitro biofilms of Streptococcus pneumoniae (Mukesh Kumar Yadav et al., 2012)
  • Used Google Scholar to search for full article
  • Article PDF involves comparing microarray data of S. pneumoniae biofilm to planktonic cells. 21 biological microarray replicates were done, with the control being the planktonic cells.
  • Searched article for location of microarray data, located here: http://www.ncbi.nlm.nih.gov/geo under

accession number GSE34751

Yadav, M. K., Kwon, S. K., Cho, C. G., Park, S.-W., Chae, S.-W. and Song, J.-J. (2012), Gene expression profile of early in vitro biofilms of Streptococcus pneumoniae. Microbiology and Immunology, 56: 621–629.

  • Based on this report and other titles listed while searching, a lot of research regarding vaccines against the pathogenic strain is popular, in addition to research on how to prevent the bacteria from growing.

[edit] Article 2:

  • Found second article using references from first article, looked for key words in title about transcription activity/microarray data
  • link to article: [1]
  • Full Citation:

Ng, WL, Kazmierczak, KM Robertson, GT, et al., 2003. Transcriptional regulation and signature patterns revealed by microarray analyses of Streptococcus pneumoniae R6 challenged with sublethal concentrations of translation inhibitors. Journal of Bacteriology. Volume 185, Issue 1, Pages 359-370.

  • In this experiment, cell cultures were exposed to 4 different translation inhibitors to determine the effect on the transcript activity. The control used was a 1% DMSO culture that contained no antibiotics. There were 3 replicates done of the microarray analysis, and Northern blot analysis was also used.

[edit] Links

Alina's User Page Kevin's User Page Tauras's User Page
Biological Databases Class Page Gene Database Project Gene Database Project Report Guidelines

Streptococcus pneumoniae

Import Export Cycle 1: tATK Export One: TIGR4 Testing Report
Import Export Cycle 2: tATK E2: TIGR4 Testing Report
Import Export Cycle 3: tATK E3: TIGR4 Testing Report
Import Export Cycle 4: tATK E4: TIGR4 Testing Report
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