Difference between revisions of "Bklein7 Week 2"

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(Wrote out the complementary DNA & associated mRNA strands)
(Preliminary Answers & Formatting)
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==Individual Journal Assignment==
 
==Individual Journal Assignment==
 
=== The Genetic Code ===
 
=== The Genetic Code ===
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+
====DNA & mRNA Sequences====
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* Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.
+
* '''DNA Strands'''
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+
 
  5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
 
  5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
 
  3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'
 
  3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'
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* There are six possible reading frames in double-stranded DNA.  Using the [http://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table genetic code], translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
 
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** In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
 
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** The genetic code is read in the 5’ to 3’ direction.
 
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** Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.
 
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* Wiki Hint:  if you start your line with a single space character, it will appear in the grey box surrounded by the dashed line as shown above.
 
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* Which of the reading frames (if any) of the reading frames you translated is an ''open reading frame'', i.e., does not contain a stop codon?
 
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** By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.
 
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−
*mRNA strands
 
  
 +
*'''mRNA Strands'''
 
  5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’
 
  5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’
 
  3'-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5'
 
  3'-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5'
 +
 +
====Reading Frames====
 +
*'''+1'''
 +
''mRNA'': 5’-cgu aug cua aua cca ugu ucc gcg uau aac cca gcc gcc agu ucc gcu ggc ggc auu uua-3’
 +
''Translation'': N-ter-R M L I P C S A Y N P A A S S A G G I L-C-ter
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*'''+2'''
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''mRNA'': 5’-c gua ugc uaa uac cau guu ccg cgu aua acc cag ccg cca guu ccg cug gcg gca uuu ua-3’
 +
''Translation'': N-ter-V C (stop)-C-ter
 +
*'''+3'''
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''mRNA'': 5’-cg uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cau uuu a-3’
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''Translation'': N-ter-Y A N T M F R V (stop)-C-ter
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*'''-1'''
 +
''mRNA'': 5'-uaa aau gcc gcc agc gga acu ggc ggc ugg guu aua cgc gga aca ugg uau uag cau acg-3'
 +
''Translation'': No protein synthesized; first codon within this reading frame is a stop codon
 +
*'''-2'''
 +
''mRNA'': 5'-u aaa aug ccg cca gcg gaa cug gcg gcu ggg uua uac gcg gaa cau ggu auu agc aua cg-3'
 +
''Translation'': N-ter-K M P P A E L A A G L Y A E H G I S I-C-ter
 +
*'''-3'''
 +
''mRNA'': 5'-ua aaa ugc cgc cag cgg aac ugg cgg cug ggu uau acg cgg aac aug gua uua gca uac g-3'
 +
''Translation'': N-ter-K C R Q R N W R L G Y T R N M V L A Y-C-ter
 +
 +
*The following reading frames were open: +1, -2, and -3.
  
 
==Links==
 
==Links==

Revision as of 06:17, 14 September 2015

Individual Journal Assignment

The Genetic Code

DNA & mRNA Sequences

  • DNA Strands
5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'
  • mRNA Strands
5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’
3'-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5'

Reading Frames

  • +1
mRNA: 5’-cgu aug cua aua cca ugu ucc gcg uau aac cca gcc gcc agu ucc gcu ggc ggc auu uua-3’
Translation: N-ter-R M L I P C S A Y N P A A S S A G G I L-C-ter
  • +2
mRNA: 5’-c gua ugc uaa uac cau guu ccg cgu aua acc cag ccg cca guu ccg cug gcg gca uuu ua-3’
Translation: N-ter-V C (stop)-C-ter
  • +3
mRNA: 5’-cg uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cau uuu a-3’
Translation: N-ter-Y A N T M F R V (stop)-C-ter
  • -1
mRNA: 5'-uaa aau gcc gcc agc gga acu ggc ggc ugg guu aua cgc gga aca ugg uau uag cau acg-3'
Translation: No protein synthesized; first codon within this reading frame is a stop codon
  • -2 
mRNA: 5'-u aaa aug ccg cca gcg gaa cug gcg gcu ggg uua uac gcg gaa cau ggu auu agc aua cg-3'
Translation: N-ter-K M P P A E L A A G L Y A E H G I S I-C-ter
  • -3
mRNA: 5'-ua aaa ugc cgc cag cgg aac ugg cgg cug ggu uau acg cgg aac aug gua uua gca uac g-3'
Translation: N-ter-K C R Q R N W R L G Y T R N M V L A Y-C-ter
  • The following reading frames were open: +1, -2, and -3.

Links

Assignments Pages

Individual Journal Entries

Shared Journal Entries

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