Marmas Week 6

Purpose

The purpose of this experiment is to develop an understanding of DNA microarray experiments and their function by answering questions concerning their analysis. Additionally, a case study will be looked at that examines a violation of the standards of research ethics.

Questions

1. 

   Question 1, based on Figure 4.6B

1. Transcriptional Response of Three genes to the gradual loss of oxygen
   • Gene X
     • Hour 1: Black
     • Hour 3: Medium Red
     • Hour 5: Black
     • Hour 9: Bright Green
   • Gene Y
     • Hour 1: Black
     • Hour 3: Bright Red
     • Hour 5: Dim Green
     • Hour 9: Bright Green
   • Gene Z
     • Hour 1: Black
     • Hour 3: Dim Red
     • Hour 5: Medium Red
     • Hour 9: Medium Red
2. Genes X and Y were transcribed very similarly, except for at hour 3. However, looking at the ratio of activity, they are very similar at each hour.
3. On a color spectrum, yellow is in between red and green. Thus, the color yellow, which on microarrays represents no change, shows that the gene is neither being induced or repressed. As it becomes more repressed, the color will shift to a shade of green. As it becomes more induced, it will shift to a shade of red. Looking at a microarray, there are some genes that show orange or a yellow-green color. This is an example of a less potent induction or repression as compared to a strong induction or strong repression. In an experiment, the gene expression may not change, thus showing a yellow spot.

Acknowledgements

• I would like to acknowledge my homework partner, David Ramirez, for aiding in the understanding of the analysis questions.
• I would like to acknowledge Dr. Dahlquist for her continuous help in the understanding of this field.