Knguye66 Week 9
Contents
Microrray Data Analysis (wild type data)
Purpose
This week's assignment is to continue the Microarray Data Anaylsis for wild type data. Step 4 and 5 from Week 9 are featured: Viewing and Saving STEM results, and Analyzing and Interpreting STEM results. For reference and files, go to: knguye66 Week 8.
Methods/Results
After finishing the steps on Week 8 for Statistical Analysis Part I: ANOVA on Microsoft Excel, a quick sanity check was performed for the p-value dataset. Following the filtering of the data, with a p-value of less than 0.05, 2528 of 6189 records were found. This means, in a p-value of less than 0.05, there is a 5% chance that 2528 out of 6189 records would be found.
Analyzing and Interpreting STEM Results
- Profile #45 -
- Why did you select this profile? In other words, why was it interesting to you?
- How many genes belong to this profile?
- How many genes were expected to belong to this profile?
- What is the p value for the enrichment of genes in this profile?
- How many GO terms are associated with this profile at p < 0.05?
- How many GO terms are associated with this profile with a corrected p value < 0.05?
- Look up the definitions for each of the terms at http://geneontology.org. In your research presentation, you will discuss the biological interpretation of these GO terms. In other words, why does the cell react to cold shock by changing the expression of genes associated with these GO terms? Also, what does this have to do with the transcription factor being deleted (for the groups working with deletion strain data)?
Data and Files
- Profile Genelist (zip file)
- Profile GOlist (zip file)
- "Interface options" & Significant Profiles (.pptx)
Conclusion
Acknowledgements
This section is in acknowledgement to partner Christina Dominguez (User:Cdomin12), as well as, Marcus Avila (User:Mavila9) and Jonar Cowan (User:Jcowan4). I would also like to acknowledge Dr. Dahlquist (User:KDahlquist) for introducing and teaching the topic and direction of this assignment.
"Except for what is noted above, this individual journal entry was completed by me and not copied from another source."
User Page
User:knguye66
Template Page
Template:knguye66
Table of all assignments and journal entries for BIO-367-01
References
- GEO Accession viewer. (n.d.). Retrieved from https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE83656.
- Dahlquist, K. (2019, October 17). Week 8. In Wikipedia, Biological Databases. Retrieved 11:14, October 21, 2019, from https://xmlpipedb.cs.lmu.edu/biodb/fall2019/index.php/Week_8