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| − | ==Presentation Prep==
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| − | ===Defining 10 unknown biological terms===
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| − | ===Questions about your article===
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| − | # '''What is the main result presented in this paper?'''
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| − | #*CHP exposure changes the transcriptional response of yeast via its oxidative stress response. The researchers observed the changes in yeast transcription from minutes 0-20, noting many significant changes in minutes 0-6.
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| − | #**Implicated pathways include oxidative phosphorylation, galactose metabolism, ATP synthesis, proteasome proteolysis, and glutathione & thioredoxin systems.
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| − | # '''What is the importance or significance of this work?'''
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| − | #*This paper was the first to note the early (0-6 minutes post-exposure) transcriptional response by yeast to CHP.
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| − | #*The researchers found that ''Yap3'', ''5'', and ''7'' are involved in the yeast OSR, and that their functions were not previously known.
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| − | # '''What were the limitations in previous studies that led them to perform this work?'''
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| − | #*Some similar studies did not have biological replicates, nor control groups.
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| − | #*This study is unique as there was no incubation with CHP before monitoring the transcriptional change. Some other studies waited 10+ minutes to begin analyzing the changes in transcription.
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| − | # '''How did they treat the yeast cells (what experiment were they doing?)'''
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| − | #*CHP, or cumene hydroperoxide, exposure (CHP in 95% (w/v) ethanol)
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| − | # '''What strain(s) of yeast did they use? Were the strain(s) haploid or diploid?'''
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| − | #*Diploid strain ''BY4743'' was used.
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| − | # What media did they grow them in? What temperature? What type of incubator? For how long?
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| − | #*2% (w/v) sucrose supplemented with uracil L-leucine, and L-histidine
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| − | #*30ºC
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| − | #*New Brunswick BioFlo fermentors
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| − | #*Grown until OD600 of 1.5
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| − | # '''What controls did they use? '''
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| − | #*The same strain of yeast was grown and monitored exactly the same way as the treatment group, but not exposed to CHP.
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| − | # '''How many replicates did they perform per treatment or timepoint?'''
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| − | #*3
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| − | # What method did they use to prepare the RNA, label it and hybridize it to the microarray?
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| − | # What mathematical/statistical method did they use to analyze the data?
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| − | # Are the data publicly available for download? From which web site?
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| − | #Briefly state the result shown in each of the figures and tables, not just the ones you are presenting.
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| − | #* What do the X and Y axes represent (if applicable)?
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| − | #* How were the measurements made?
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| − | #* What trends are shown by the plots and what conclusions can you draw from the data?
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| − | # How does this work compare with previous studies?
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| − | # What are the important implications of this work?
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| − | # What future directions should the authors take?
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| − | # Give a critical evaluation of how well you think the authors supported their conclusions with the data they showed. Are there any major flaws to the paper?
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| | ==Presentation== | | ==Presentation== |
| | [[Media:Hailey_Charlotte_Katie_Journal_Club_Presentation.pdf|Ivanson, Kaplan, Miller on Sha et al., 2013]] | | [[Media:Hailey_Charlotte_Katie_Journal_Club_Presentation.pdf|Ivanson, Kaplan, Miller on Sha et al., 2013]] |
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| − | ==References==
| + | {{Hivanson}} |
