Methods/Results/Electronic Notebook

Purpose

The purpose of this assignment is to get experience with the analysis part of the "data life cycle" for DNA microarray datasets and to "develop an intuition about different p-value cut-offs and their meanings.

It also is to show how to keep a detailed electronic lab notebook to make our research reproducible to develop an intuition about what different p-value cut-offs mean.

Methods/Electronic Notebook

1) I inserted a new worksheet into my Excel workbook and named it dGLN3_stem.

2) Selected all of the data from my dGLN3_ANOVA worksheet and pasted it as values into my new dGLN3_stem worksheet.

3) Renamed my left MasterIndex column to SPOT

4)Renamed Column B to "Gene Symbol". Deleted the column named "Standard_Name". Filtered the data on the B-H corrected p value to be > 0.05. Selected all of the rows (except for the header) and deleted the rows by right-clicking and choosing "Delete Row" from the context menu. Undid the filter.

Data and Files

Media:AS&CK_BIOL367_S24_microarray-data_dGLN3.xlsx

Media:BIOL367_S24_Andrew_dGLN3_p-value_slide.pptx

Media:AS&CK_BIOL367_STEM.txt

Conclusion

I am not sure we have concluded this task yet. I think the conclusion will come with part 2.

Acknowledgements & References

Acknowledgments

I worked on this assignment with Charlotte Kaplan in class. I helped her with a lot of her excel stuff because I had more experience. I texted her at 5:57 pm on wednesday about the p-values for B&H being strangely low for me. For the most part I did the assignment on my own and really was helping her catch up to me.
Dr. Dahlquist was very helpful in class when I ran into some errors with the data. The specific errors are in the electronic notebook.
I used the Week 9 instructions and followed them.
- Except for what is noted above, this individual journal entry was completed by me and not copied from another source.

18:04, 20 March 2024 (PDT)

References

Dahlquist, K. Master_sheet_dGLN3.
LMU BioDB 2024. (2024). Week 9. Retrieved Mar 20, 2024, from https://xmlpipedb.cs.lmu.edu/biodb/Spring2024/index.php/Week_9

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