Cdomin12 Week 2

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template: cdomin12

Assignment Page	Individual Journal Entries	Class Journal
Week 1	cdomin12 Week 1	Class Journal Week 1
Week 2	cdomin12 Week 2	Class Journal Week 2
Week 3	RAD53 / YPL153C Week 3	Class Journal Week 3
Week 4	cdomin12 Week 4	Class Journal Week 4
Week 5	IMG/VR Week 5	Class Journal Week 5
Week 6	cdomin12 Week 6	Class Journal Week 6
Week 7	cdomin12 Week 7	Class Journal Week 7
Week 8	cdomin12 Week 8	Class Journal Week 8
Week 9	cdomin12 Week 9	Class Journal Week 9
Week 10	cdomin12 Week 10	Class Journal Week 10
Week 11	cdomin12 Week 11	Skinny Genes
Week 12/13	Skinny Genes Quality Assurance	Skinny Genes
Week 15	Skinny Genes Deliverables	Skinny Genes

Purpose

The purpose of the investigations was to uncover more about the different mechanisms that relate to flower color. When looking at it from a genetic, biochemical, molecular biology, and evolutionary point of view, there are a lot of factors that go into making any characteristic. It can give us a deeper understanding and appreciation for biological processes.

Methods/Results

Methods done according to Part I: Genetics

Part 1: Genetics-Crosses

(a) and (b)

C^W - white (recessive)

C^Y - yellow

C^G - green (dominant)

C^B - blue

C^R - red

(c)

C^GC and C^BC^Y - green

C^WC^W - white

C^GC^R - black

C^R C^B - purple

C^RC^Y - orange

C^RC^R and C^RC^W- red

C^BC^B and C^BC^W- blue

C^YC^Y and C^YC^W - yellow

(d)

Green 1: C^GC

Green 2: C^BC^Y

Red: C^RC

White: C^WC^W

(e)

Purple: C^RC^B

(f)

You would not be able to construct a pure breeding purple flower since it only results from codominance of the red and blue allele and thus will never be true breeding.

Part 2: Genetics-Mutations

The starting color was blue and the mutating color was purple.

There is a change is a deletion of 10 amino acids, marked with a red (*), that result from the mutation in the pure bred blue.

There is a deletion in a single cysteine nucleotide marked with a red (*).

Scientific Conclusion

The green allele was dominant over all other alleles, while the white allele was recessive to all others. There multiple occurrences of codominance including the color purple, black, orange, and green-2. When a mutation occurred in a true breeding blue flower, it caused a single deletion of a cysteine. Even though it was only a single nucleotide, it caused a significant change in the amino acid sequence causing the color change from blue to purple. It is clear that there are many alleles that go into flower color. Mutations can cause amino acid changes which ultimately affect protein shape and binding.

Data/Files

The file was not able to be uploaded due to multiple formatting issues when changing to different versions. I have it saved on my computer and flash drive. This is a photo of the purple mutated organism from the pure blue flower.

Acknowledgements

1. I worked with user:dmadere on this assignment. We worked in class together on genotypes and phenotypes in part 1 and also texted outside of class for part 2 of the assignment.
2. I used Manual to code how to use exponents.
3. "Except for what is noted above, this individual journal entry was completed by me and not copied from another source."

Cdomin12 (talk) 21:57, 10 September 2019 (PDT)

References

• Aipotu. (2017). Aipotu. Retrieved September 10, 2019, from http://aipotu.umb.edu/
• LMU BioDB 2019. (2019). Week 2. Retrieved September 10, 2019, from https://xmlpipedb.cs.lmu.edu/biodb/fall2019/index.php/Week_2