Difference between revisions of "Msamdars Week 2"
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===Results=== | ===Results=== | ||
| + | Changes from white allele are highlighted. | ||
====Tasks A & B==== | ====Tasks A & B==== | ||
| − | {| class="wikitable" | + | {| class="wikitable" style="width: 85%;" |
!Allele | !Allele | ||
!Color | !Color | ||
| Line 19: | Line 20: | ||
!Change(s) in DNA Sequence | !Change(s) in DNA Sequence | ||
|- | |- | ||
| − | | | + | |B |
|Blue | |Blue | ||
| − | | | + | |<code>CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTG<u style="color: red">'''TA'''</u>CTGTCGGCAGTAGTAGGGGGCGT</code> |
| − | | | + | |<code>N-MetSerAsnArgHisIleLeuLeuVal<u style="color: red">'''Tyr'''</u>CysArgGln-C</code> |
| + | |- | ||
| + | |R | ||
| + | |Red | ||
| + | |<code>CAGCTAT<u style="color: red">'''A'''</u>ACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGTTCTGTCGGCAGTAGTAGGGGGCGT</code> | ||
| + | |<code>N-MetSerAsnArgHisIleLeuLeuValPheCysArgGln-C</code> | ||
| + | |- | ||
| + | |G | ||
| + | |Green | ||
| + | |<code>CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGTACTGGCGGCAGTAGTAGGGGGCGT</code> | ||
| + | |<code>N-MetSerAsnArgHisIleLeuLeuValTyrTrpArgGln-C</code> | ||
| + | |- | ||
| + | |Y | ||
| + | |Yellow | ||
| + | |<code>CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGTGGTGTCGGCAGTAGTAGGGGGCGT</code> | ||
| + | |<code>N-MetSerAsnArgHisIleLeuLeuValTrpCysArgGln-C</code> | ||
| + | |- | ||
| + | |W | ||
| + | |White | ||
| + | |<code>CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGGTCTGTCGGCAGTAGTAGGGGGCGT</code> | ||
| + | |<pre>N-MetSerAsnArgHisIleLeuLeuValValCysArgGln-C</code> | ||
|} | |} | ||
====Task C==== | ====Task C==== | ||
| Line 28: | Line 49: | ||
====Task D==== | ====Task D==== | ||
| + | |||
| + | Tyrosine Phenylalanine Tryptophan added make purple. | ||
| + | Because both the | ||
====Task E==== | ====Task E==== | ||
Revision as of 20:50, 10 September 2019
Contents
Electronic Lab Notebook
Purpose
The purpose of this experiment is to learn about models and modeling, as well as the central dogma of molecular biology by utilizing the software Aipotu. We will use this software to learn about the real computational work that systems biologists do and emulate that work with hypothetical organisms as a control.
Methods
Task A
- Loaded each allele in to Aipotu
Task B
- Loaded sample by double-clicking on White alleles (as pictured).
- Compared sequence using compare tool
Results
Changes from white allele are highlighted.
Tasks A & B
| Allele | Color | Change(s) in Amino Acid Sequence | Change(s) in DNA Sequence |
|---|---|---|---|
| B | Blue | CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGTACTGTCGGCAGTAGTAGGGGGCGT
|
N-MetSerAsnArgHisIleLeuLeuValTyrCysArgGln-C
|
| R | Red | CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGTTCTGTCGGCAGTAGTAGGGGGCGT
|
N-MetSerAsnArgHisIleLeuLeuValPheCysArgGln-C
|
| G | Green | CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGTACTGGCGGCAGTAGTAGGGGGCGT
|
N-MetSerAsnArgHisIleLeuLeuValTyrTrpArgGln-C
|
| Y | Yellow | CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGTGGTGTCGGCAGTAGTAGGGGGCGT
|
N-MetSerAsnArgHisIleLeuLeuValTrpCysArgGln-C
|
| W | White | CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGGTCTGTCGGCAGTAGTAGGGGGCGT
|
N-MetSerAsnArgHisIleLeuLeuValValCysArgGln-C |
Task C
Given the screenshot on the right, there were no differences in the white alleles.
Task D
Tyrosine Phenylalanine Tryptophan added make purple. Because both the
Task E
Task F
Conclusion
Other Links
| Links | ||
|---|---|---|
| Mihir Samdarshi User Page | ||
| Assignment Pages | Personal Journal Entries | Shared Journal Entries |
| Week 1 | Journal Week 1 | Class Journal Week 1 |
| Week 2 | Journal Week 2 | Class Journal Week 2 |
| Week 3 | FAS2/YPL231W Week 3 | Class Journal Week 3 |
| Week 4 | Journal Week 4 | Class Journal Week 4 |
| Week 5 | Database - AmtDB | Class Journal Week 5 |
| Week 6 | Journal Week 6 | Class Journal Week 6 |
| Week 7 | Journal Week 7 | Class Journal Week 7 |
| Week 8 | Journal Week 8 | Class Journal Week 8 |
| Week 9 | Journal Week 9 | Class Journal Week 9 |
| Assignment Pages | Personal Journal Entries | |
| Week 10 | Journal Week 10 | |
| Week 11 | Journal Week 11 | |
| Week 12/13 | Journal Week 12/13 | |
| Team Project Links | ||
| Skinny Genes Team Page | ||
Acknowledgement
This week my partner was John Nimmers-Minor. Except for what is noted above, this individual journal entry was completed by me and not copied from another source.
References
# LMU BioDB 2019. (2019). Week 2. Retrieved September 5, 2019, from https://xmlpipedb.cs.lmu.edu/biodb/fall2019/index.php/Week_2
