Difference between revisions of "Cdomin12 Week 11"
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− | 1. I worked with [[User:ymesfin]], [[User:Jcowan4]], [[User: | + | 1. I worked with [[User:ymesfin]], [[User:Jcowan4]], [[User:msamdars]], and [[User:dramir36]] for this assignment. |
2."Except for what is noted above, this individual journal entry was completed by me and not copied from another source." | 2."Except for what is noted above, this individual journal entry was completed by me and not copied from another source." |
Revision as of 19:54, 11 November 2019
Contents
- 1 1. Ten Biological Terms
- 2 2. Outline of Article
- 2.1 1. What is the main result presented in this paper?
- 2.2 2. What is the importance or significance of this work?
- 2.3 3. What were the limitations in previous studies that led them to perform this work?
- 2.4 4. How did they treat the yeast cells (what experiment were they doing?)
- 2.5 5. What strain(s) of yeast did they use? Were the strain(s) haploid or diploid?
- 2.6 6. What media did they grow them in? What temperature? What type of incubator? For how long?
- 2.7 7. What controls did they use?
- 2.8 8. How many replicates did they perform per treatment or timepoint?
- 2.9 9. What method did they use to prepare the RNA, label it and hybridize it to the microarray?
- 2.10 10. What mathematical/statistical method did they use to analyze the data?
- 2.11 11. Are the data publicly available for download? From which web site?
- 2.12 12. Briefly state the result shown in each of the figures and tables, not just the ones you are presenting.
- 2.13 13. What do the X and Y axes represent?
- 2.14 14. How were the measurements made?
- 2.15 15. What trends are shown by the plots and what conclusions can you draw from the data?
- 2.16 16. How does this work compare with previous studies?
- 2.17 17. What are the important implications of this work?
- 2.18 18. What future directions should the authors take?
- 2.19 19. Give a critical evaluation of how well you think the authors supported their conclusions with the data they showed. Are there any major flaws to the paper?
- 3 Acknowledgments
- 4 References
1. Ten Biological Terms
- septin: a protein involved in cytokinesis and also functions in maintaining cell morphology (https://www.biology-online.org/dictionary/Septin, Biology Online, 2019)
- retrograde: Moving backward or against the usual direction of flow (https://www.biology-online.org/dictionary/Retrograde, Biology Online, 2019)
- assimilation:The conversion of nutriment into a useable form (e.g. liquid or solid) that is incorporated into the tissues and organs following the processes of digestion. (https://www.biology-online.org/dictionary/Assimilation, Biology Online, 2019)
- attenuated: to reduce the virulence (infectivity) of a pathogenic microorganism.(https://www.biology-online.org/dictionary/Attenuated, Biology Online, 2019)
- autotrophy: The state of being self-sustaining and being able to produce food from inorganic compounds.(https://www.biology-online.org/dictionary/Autotrophy, Biology Online, 2019)
- trehalose:disaccharide carbohydrate. (https://www.biology-online.org/dictionary/Trehalose, Biology Online, 2019)
- homocysteine: amino acid cysteine (https://www.biology-online.org/dictionary/Main_Page, Biology Online, 2019)
- chemostat:experimental apparatus where the chemical environment can be maintained static and nutrient availability can be controlled by the experimenter.(https://www.sciencedirect.com/topics/neuroscience/chemostat, ScienceDirect, 2019)
- methylglyoxal: organic compound; reduced derivate of pyruvic acid (https://www.biology-online.org/dictionary/Main_Page, Biology Online, 2019)
- Trk1: potassium transport protein (http://amigo.geneontology.org/amigo/search/bioentity?q=trk1, GeneOntology, 2019)
2. Outline of Article
1. What is the main result presented in this paper?
2. What is the importance or significance of this work?
3. What were the limitations in previous studies that led them to perform this work?
4. How did they treat the yeast cells (what experiment were they doing?)
5. What strain(s) of yeast did they use? Were the strain(s) haploid or diploid?
6. What media did they grow them in? What temperature? What type of incubator? For how long?
7. What controls did they use?
8. How many replicates did they perform per treatment or timepoint?
9. What method did they use to prepare the RNA, label it and hybridize it to the microarray?
10. What mathematical/statistical method did they use to analyze the data?
11. Are the data publicly available for download? From which web site?
12. Briefly state the result shown in each of the figures and tables, not just the ones you are presenting.
Figure 4: (A) This demonstrates the effect of methylglyoxal production as well as trehalose metabolism from potassium starvation. (B) Two graphs (x axis- time in min and y axis- changes in expression fold) shows the change in expression specifically for genes related to trehalose metabolism and methylglyoxal. Measurements were made through the microarray data. (C) A graph (x axis- time in min and y axis- methylglyoxal concentration) showing MG levels remaining higher after 20 minutes potentially linked to genes induced in trehalose metabolism.
13. What do the X and Y axes represent?
14. How were the measurements made?
15. What trends are shown by the plots and what conclusions can you draw from the data?
16. How does this work compare with previous studies?
17. What are the important implications of this work?
18. What future directions should the authors take?
19. Give a critical evaluation of how well you think the authors supported their conclusions with the data they showed. Are there any major flaws to the paper?
Acknowledgments
1. I worked with User:ymesfin, User:Jcowan4, User:msamdars, and User:dramir36 for this assignment.
2."Except for what is noted above, this individual journal entry was completed by me and not copied from another source." Cdomin12 (talk) 19:53, 11 November 2019 (PST)
References
- Biology Online. 2019. Accessed November 11, 2019, from https://biology-online.org
- GeneOntology. 2019. Accessed November 11, 2019, from http://geneontology.org
- ScienceDirect. 2019. Accessed November 11, 2019, from https://www.sciencedirect.com/topics/neuroscience/chemostat
- Week 11. Retrieved November 11, 2019, from https://xmlpipedb.cs.lmu.edu/biodb/fall2019/index.php/Week_10