Dramir36 Week 7

User:Dramir36 template:Dramir36 Skinny Genes

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Purpose

• to conduct the "analyze" step of the data life cycle for a DNA microarray dataset.

• to develop an intuition about what different p-value cut-offs mean.

• to keep a detailed electronic laboratory notebook to facilitate reproducible research.

• to revisit the "Deception at Duke" case with new insights because you have analyzed your own dataset.

Methods/Results

• T test: is this gene expression change significantly different than zero at a time point?

p>0.05 5%

probability that you would have seen at least this big of a change by chance.

• ANOVA: is the gene expression significantly different than zero at any time point?

• Values below 0.25 should be considered to be a gene with no change in expression