Ckaplan Week 12
Media:Hailey_Charlotte_Katie_Journal_Club_Presentation.pdf
- What is the main result presented in this paper?
The paper shows how Saccharomyces cerevisiae responds in the first 20 minutes to oxidative stress caused by cumene hydroperoxide (CHP). Using experiments with time controls and duplicate samples, the study finds new genes involved in this stress response and highlights differences in how cells react to CHP compared to hydrogen peroxide. The study used the data only up to t 20, highlighting how after 20 minutes, the yeast exhibited a stress response.
- What is the importance or significance of this work?
This research is important because it's the first to show how yeast quickly responds (within 0-6 minutes) to CHP-induced stress. It also uncovers the roles of Yap3, Yap5, and Yap7 in yeast's response to oxidative stress, which weren't known before. Understanding these processes better could help us grasp how cells deal with stress and how it relates to diseases.
- What were the limitations in previous studies that led them to perform this work?
Previous studies didn't use control groups and incubated the CHP. Because of these issues, it was hard to understand the data well. This study tried to fix these problems by keeping track of time, using enough samples, and controlling environmental factors tightly to get a better understanding of how cells respond early to CHP-induced stress.
- How did they treat the yeast cells (what experiment were they doing?)
CHP- cumene hydroperoxide.
- What strain(s) of yeast did they use? Were the strain(s) haploid or diploid?
Diploid- BY4743.
- What media did they grow them in? What temperature? What type of incubator? For how long?
2% (w/v) sucrose with uracil L-leucine, and L-histidine at 30ºC using BioFlo fermentors.
- What controls did they use?
Yeast strain BY4743 was used but was not exposed to CHP.
- How many replicates did they perform per treatment or timepoint?
3
- What method did they use to prepare the RNA, label it and hybridize it to the microarray?
- What mathematical/statistical method did they use to analyze the data?
- Are the data publicly available for download? From which web site?
- Briefly state the result shown in each of the figures and tables, not just the ones you are presenting.
- What do the X and Y axes represent (if applicable)?
- How were the measurements made?
- What trends are shown by the plots and what conclusions can you draw from the data?
- How does this work compare with previous studies?
- What are the important implications of this work?
- What future directions should the authors take?
- Give a critical evaluation of how well you think the authors supported their conclusions with the data they showed. Are there any major flaws to the paper?
Assignment Pages
- Week 1
- Week 2
- Week 3
- Week 4
- Week 5
- Week 6
- Week 8
- Week 9
- Week 10
- Week 11
- Week 12
- Week 13
- Week 14
- Week 15
Individual Journal Entry Pages
- ckaplan Week 1
- ckaplan Week 2
- SIR2 Week 3
- AgeAnnoMO Week 4
- ckaplan Week 5
- ckaplan Week 6
- ckaplan Week 8
- ckaplan Week 9
- ckaplan Week 10
- ckaplan Week 11
- ckaplan Week 12
- ckaplan Week 13
- ckaplan Week 14
- ckaplan Week 15
